ERF mutations reveal a balance of ETS factors controlling prostate oncogenesis

Rohit Bose, Wouter R. Karthaus, Joshua Armenia, Wassim Abida, Phillip J. Iaquinta, Zeda Zhang, John Wongvipat, Elizabeth V. Wasmuth, Neel Shah, Patrick S. Sullivan, Michael G. Doran, Ping Wang, Anna Patruno, Yilin Zhao, Deyou Zheng, Nikolaus Schultz, Charles L. Sawyers

Resultado de la investigación: Articlerevisión exhaustiva

51 Citas (Scopus)


Half of all prostate cancers are caused by the TMPRSS2-ERG gene-fusion, which enables androgens to drive expression of the normally silent E26 transformation-specific (ETS) transcription factor ERG in prostate cells1-2. Recent genomic landscape studies of such cancers3-8 have reported recurrent point mutations and focal deletions of another ETS member, the ETS2 repressor factor ERF. Here we show these ERF mutations cause decreased protein stability and mostly occur in tumours without ERG upregulation. ERF loss recapitulates the morphological and phenotypic features of ERG gain in normal mouse prostate cells, including expansion of the androgen receptor transcriptional repertoire, and ERF has tumour suppressor activity in the same genetic background of Pten loss that yields oncogenic activity by ERG. In the more common scenario of ERG upregulation, chromatin immunoprecipitation followed by sequencing indicates that ERG inhibits the ability of ERF to bind DNA at consensus ETS sites both in normal and in cancerous prostate cells. Consistent with a competition model, ERF overexpression blocks ERG-dependent tumour growth, and ERF loss rescues TMPRSS2-ERG-positive prostate cancer cells from ERG dependency. Collectively, these data provide evidence that the oncogenicity of ERG is mediated, in part, by competition with ERF and they raise the larger question of whether other gain-of-function oncogenic transcription factors might also inactivate endogenous tumour suppressors.

Idioma originalEnglish (US)
Páginas (desde-hasta)671-675
Número de páginas5
EstadoPublished - jun. 29 2017
Publicado de forma externa

ASJC Scopus subject areas

  • General


Profundice en los temas de investigación de 'ERF mutations reveal a balance of ETS factors controlling prostate oncogenesis'. En conjunto forman una huella única.

Citar esto