Epigenetic reprogramming of mesenchymal stem cells

Yu Wei Leu, Tim H.M. Huang, Shu Huei Hsiao

Producción científica: Chapter

16 Citas (Scopus)

Resumen

Mesenchymal stem cells (MSCs) are multipotent stem cells of mesodermal origin that can be isolated from various sources and induced into different cell types. Although MSCs possess immune privilege and are more easily obtained than embryonic stem cells, their propensity to tumorigenesis has not been fully explored. Epigenomic changes in DNA methylation and chromatin structure have been hypothesized to be critical in the determination of lineage-specific differentiation and tumorigenesis of MSCs, but this has not been formally proven. We applied a targeted DNA methylation method to methylate a Polycomb group protein-governed gene, Trip10, in MSCs, which accelerated the cell fate determination of MSCs. In addition, targeted methylation of HIC1 and RassF1A, both tumor suppressor genes, transformed MSCs into tumor stem cell-like cells. This new method will allow better control of the differentiation of MSCs and their use in downstream applications.

Idioma originalEnglish (US)
Título de la publicación alojadaEpigenetic Alterations in Oncogenesis
EditorialSpringer Science and Business Media, LLC
Páginas195-211
Número de páginas17
ISBN (versión impresa)9781441999665
DOI
EstadoPublished - 2013

Serie de la publicación

NombreAdvances in Experimental Medicine and Biology
Volumen754
ISSN (versión impresa)0065-2598

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology

Huella

Profundice en los temas de investigación de 'Epigenetic reprogramming of mesenchymal stem cells'. En conjunto forman una huella única.

Citar esto