@article{c40da1d479a14788b4d684fafbaca8ca,
title = "Electrophoresis of proteins and nucleic acids on acrylamide-agarose gels lacking covalent crosslinking",
abstract = "The preparation of acrylamide-agarose gels lacking covalent crosslinking with methylenebis-acrylamide is described. These hybrid gels melt at 85°C and, consequently, allow quantitative analysis of tritium-labeled protein after electrophoresis. Recovery of tritium-labeled ribonucleic acids extracted from hybrid gels is 20 to 25% greater than from standard acrylamide-methylenebisacrylamide gels. Standard curves of electrophoretic mobilities as a function of molecular weights of dissociated proteins and ribonucleic acids are compared for acrylamide-agarose gels and acrylamide-methylenebisacrylamide gels.",
keywords = "acrylamide, agarose, gel electrophoresis, nucleic acids, proteins",
author = "Horowitz, {Paul M.} and Lee, {John C} and Williams, {Geri A.} and Williams, {Robert F.} and Barnes, {Larry D.}",
note = "Funding Information: We thank Angela K. Robinson for assistancein iso- lation of tubulin and diadenosine tetraphosphate pyro-phosphohydrolase and Jean Floyd and Connie Mumford for assistance in photolabeling tubulin. Dr. Neal C. Robinson and Dr. Philip Serwer kindly donated “C-methylated proteins and HGT(P) and HEEO agaroses, respectively. This research was supported by National Institutes of Health Grants GM27220 (L.D.B.), GM25 177 (P.M.H.), and GM30665 (R.F.W.), and Robert A. Welch Foundation Grants AQ-723 (P.M.H.), AX-769 (R.F.W.), and AQ-774 (L.D.B.).",
year = "1984",
month = dec,
doi = "10.1016/0003-2697(84)90672-9",
language = "English (US)",
volume = "143",
pages = "333--340",
journal = "Analytical Biochemistry",
issn = "0003-2697",
publisher = "Academic Press Inc.",
number = "2",
}