Effects of aflatoxin B1 on polysomal profiles and RNA synthesis in rat liver

Arun K. Roy

doi:10.1016/0005-2787(68)90021-X

Effects of aflatoxin B₁ on polysomal profiles and RNA synthesis in rat liver

Arun K. Roy

Resultado de la investigación: Article › revisión exhaustiva

53 Citas (Scopus)

Resumen

1. Aflatoxin B₁, a known hepatocarcinogen, is found to induce polysomal disaggregation in rat liver. 2. The entry of [¹⁴C]orotic acid into liver RNA is also inhibited by this toxin. A significant inhibition is obtained at a dose as low as 6.5 μg per 100 g body weight and the inhibition seems to be almost complete at 150 μg per 100 g body weight. At lower doses the ribosomal RNA's showed greater susceptibility to inhibition than the smaller RNA species. 3. Aflatoxin B₁ did not inhibit an in vitro RNA synthesizing system containing rat-testicular RNA polymerase (nucleoside triphosphate: RNA nucleotidyltransferase, EC 2.7.7.6). 4. These results suggest that a metabolite of aflatoxin B₁ rather than the toxin itself is a binding inhibitor of DNA-dependent RNA synthesis.

Idioma original	English (US)
Páginas (desde-hasta)	206-211
Número de páginas	6
Publicación	BBA Section Nucleic Acids And Protein Synthesis
Volumen	169
N.º	1
DOI	https://doi.org/10.1016/0005-2787(68)90021-X
Estado	Published - nov 20 1968
Publicado de forma externa	Sí

ASJC Scopus subject areas

Medicine(all)

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10.1016/0005-2787(68)90021-X

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title = "Effects of aflatoxin B1 on polysomal profiles and RNA synthesis in rat liver",

abstract = "1. Aflatoxin B1, a known hepatocarcinogen, is found to induce polysomal disaggregation in rat liver. 2. The entry of [14C]orotic acid into liver RNA is also inhibited by this toxin. A significant inhibition is obtained at a dose as low as 6.5 μg per 100 g body weight and the inhibition seems to be almost complete at 150 μg per 100 g body weight. At lower doses the ribosomal RNA's showed greater susceptibility to inhibition than the smaller RNA species. 3. Aflatoxin B1 did not inhibit an in vitro RNA synthesizing system containing rat-testicular RNA polymerase (nucleoside triphosphate: RNA nucleotidyltransferase, EC 2.7.7.6). 4. These results suggest that a metabolite of aflatoxin B1 rather than the toxin itself is a binding inhibitor of DNA-dependent RNA synthesis.",

author = "Roy, {Arun K.}",

note = "Funding Information: I gratefully acknowledge the encouragement and support of Dr. A. E. AXEL-ROD for this work. I am indebted to Dr. C. W. HESSELTINE for his generous supply of crystalline aflatoxin B 1. I thank Dr. KIVlE M~OLDAVE and Dr. DAISUKE NAKADA for the review of the manuscript. This investigation was supported by U.S. Public Health Service research grant AM-oo727-I3.",

year = "1968",

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doi = "10.1016/0005-2787(68)90021-X",

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AU - Roy, Arun K.

N1 - Funding Information: I gratefully acknowledge the encouragement and support of Dr. A. E. AXEL-ROD for this work. I am indebted to Dr. C. W. HESSELTINE for his generous supply of crystalline aflatoxin B 1. I thank Dr. KIVlE M~OLDAVE and Dr. DAISUKE NAKADA for the review of the manuscript. This investigation was supported by U.S. Public Health Service research grant AM-oo727-I3.

PY - 1968/11/20

Y1 - 1968/11/20

N2 - 1. Aflatoxin B1, a known hepatocarcinogen, is found to induce polysomal disaggregation in rat liver. 2. The entry of [14C]orotic acid into liver RNA is also inhibited by this toxin. A significant inhibition is obtained at a dose as low as 6.5 μg per 100 g body weight and the inhibition seems to be almost complete at 150 μg per 100 g body weight. At lower doses the ribosomal RNA's showed greater susceptibility to inhibition than the smaller RNA species. 3. Aflatoxin B1 did not inhibit an in vitro RNA synthesizing system containing rat-testicular RNA polymerase (nucleoside triphosphate: RNA nucleotidyltransferase, EC 2.7.7.6). 4. These results suggest that a metabolite of aflatoxin B1 rather than the toxin itself is a binding inhibitor of DNA-dependent RNA synthesis.

AB - 1. Aflatoxin B1, a known hepatocarcinogen, is found to induce polysomal disaggregation in rat liver. 2. The entry of [14C]orotic acid into liver RNA is also inhibited by this toxin. A significant inhibition is obtained at a dose as low as 6.5 μg per 100 g body weight and the inhibition seems to be almost complete at 150 μg per 100 g body weight. At lower doses the ribosomal RNA's showed greater susceptibility to inhibition than the smaller RNA species. 3. Aflatoxin B1 did not inhibit an in vitro RNA synthesizing system containing rat-testicular RNA polymerase (nucleoside triphosphate: RNA nucleotidyltransferase, EC 2.7.7.6). 4. These results suggest that a metabolite of aflatoxin B1 rather than the toxin itself is a binding inhibitor of DNA-dependent RNA synthesis.

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