Effect of prolonged storage of seminal plasma on levels of calcium and magnesium

Background: In laboratories, the semen or seminal plasma samples are stored before estimation of different elements without knowing the adverse effect of storing. The present study shows the levels of Ca⁺⁺ and Mg⁺⁺ in the seminal plasma before and after the storage. Methods: Atomic absorption spectrophotometry (AAS) was used for estimating calcium and magnesium levels in the seminal plasma. Samples were stored for one year in different containers such as polyethylene, plastic, ordinary glass, and borosil glass. Calcium and magnesium levels were estimated after this period by AAS. The total area in contact with containers was considered in the final calculation. Results: After 1-year storage, the levels of calcium and magnesium had changed. The most suitable container for storage of calcium was ordinary glass (P > 0.05) whereas ordinary glass (P > 0.05), polyethylene (P > 0.05), and borosil glass (P > 0.05) are the most suitable for magnesium. Conclusion: In any elemental study, the most suitable containers are recommended for storage of the semen/seminal plasma samples.