Doslidzhennia ekspresiï dezoksyrybonukleaz u klitynakh ssavtsiv metodom renaturatsiï bilkiv u poliakrylamidnomu heli.

I. I. Kit; H. I. Shuvaieva; V. R. Drel'; N. I. Ihumentseva; L. B. Drobot

Doslidzhennia ekspresiï dezoksyrybonukleaz u klitynakh ssavtsiv metodom renaturatsiï bilkiv u poliakrylamidnomu heli.

I. I. Kit, H. I. Shuvaieva, V. R. Drel', N. I. Ihumentseva, L. B. Drobot

Resultado de la investigación: Article › revisión exhaustiva

Resumen

Analysis of enzymatic activity in polyacrylamide gel is based on highly effective separation of proteins by SDS-electrophoresis with their subsequent renaturation and detection of enzymatic activity. This method was used to study an expression of DNAases in culturing of cells HEK293, NIH 3T3, U937. We have found that in HEK293 cells the nucleases with molecular weights 47 and 45 kDa were expressed. The localization of DNAases in the cell nuclei was shown as well. Induction of apoptosis in HEK293 cells increase the level of p47 DNAase and causes the expression of novel 50 kDa DNAase. We suggested that those discovered DNAases could take part in apoptotic DNA degradation.

Título traducido de la contribución	Study of expression of desoxyribonucleases in mammalian cells by a protein renaturation method in polyacrylamide gel
Idioma original	Ukrainian
Páginas (desde-hasta)	116-119
Número de páginas	4
Publicación	Ukrainski¿ biokhimicheski¿ zhurnal
Volumen	74
N.º	3
Estado	Published - 2002
Publicado de forma externa	Sí

ASJC Scopus subject areas

Biochemistry

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title = "Doslidzhennia ekspresi{\"i} dezoksyrybonukleaz u klitynakh ssavtsiv metodom renaturatsi{\"i} bilkiv u poliakrylamidnomu heli.",

abstract = "Analysis of enzymatic activity in polyacrylamide gel is based on highly effective separation of proteins by SDS-electrophoresis with their subsequent renaturation and detection of enzymatic activity. This method was used to study an expression of DNAases in culturing of cells HEK293, NIH 3T3, U937. We have found that in HEK293 cells the nucleases with molecular weights 47 and 45 kDa were expressed. The localization of DNAases in the cell nuclei was shown as well. Induction of apoptosis in HEK293 cells increase the level of p47 DNAase and causes the expression of novel 50 kDa DNAase. We suggested that those discovered DNAases could take part in apoptotic DNA degradation.",

author = "Kit, {I. I.} and Shuvaieva, {H. I.} and Drel', {V. R.} and Ihumentseva, {N. I.} and Drobot, {L. B.}",

year = "2002",

language = "Ukrainian",

volume = "74",

pages = "116--119",

journal = "Ukrainski¿ biokhimicheski¿ zhurnal",

issn = "2409-4943",

publisher = "Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine (NASU)",

number = "3",

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TY - JOUR

T1 - Doslidzhennia ekspresiï dezoksyrybonukleaz u klitynakh ssavtsiv metodom renaturatsiï bilkiv u poliakrylamidnomu heli.

AU - Kit, I. I.

AU - Shuvaieva, H. I.

AU - Drel', V. R.

AU - Ihumentseva, N. I.

AU - Drobot, L. B.

PY - 2002

Y1 - 2002

N2 - Analysis of enzymatic activity in polyacrylamide gel is based on highly effective separation of proteins by SDS-electrophoresis with their subsequent renaturation and detection of enzymatic activity. This method was used to study an expression of DNAases in culturing of cells HEK293, NIH 3T3, U937. We have found that in HEK293 cells the nucleases with molecular weights 47 and 45 kDa were expressed. The localization of DNAases in the cell nuclei was shown as well. Induction of apoptosis in HEK293 cells increase the level of p47 DNAase and causes the expression of novel 50 kDa DNAase. We suggested that those discovered DNAases could take part in apoptotic DNA degradation.

AB - Analysis of enzymatic activity in polyacrylamide gel is based on highly effective separation of proteins by SDS-electrophoresis with their subsequent renaturation and detection of enzymatic activity. This method was used to study an expression of DNAases in culturing of cells HEK293, NIH 3T3, U937. We have found that in HEK293 cells the nucleases with molecular weights 47 and 45 kDa were expressed. The localization of DNAases in the cell nuclei was shown as well. Induction of apoptosis in HEK293 cells increase the level of p47 DNAase and causes the expression of novel 50 kDa DNAase. We suggested that those discovered DNAases could take part in apoptotic DNA degradation.

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AN - SCOPUS:0642344230

SN - 2409-4943

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JO - Ukrainski¿ biokhimicheski¿ zhurnal

JF - Ukrainski¿ biokhimicheski¿ zhurnal

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ER -

Doslidzhennia ekspresiï dezoksyrybonukleaz u klitynakh ssavtsiv metodom renaturatsiï bilkiv u poliakrylamidnomu heli.

Resumen

ASJC Scopus subject areas

Otros archivos y enlaces

Huella

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