DNA-looping by RXR tetramers permits transcriptional regulation "at a distance"

Rubina Yasmin, Kay T. Yeung, Richard H. Chung, Maria E. Gaczynska, Pawel A. Osmulski, Noa Noy

Resultado de la investigación: Articlerevisión exhaustiva

31 Citas (Scopus)

Resumen

RXR, a member of the superfamily of nuclear hormone receptors, regulates gene transcription in response to 9-cis-retinoic acid. We previously showed that, among nuclear receptors, RXR is unique in that it self-associates into homotetramers, and that these tetramers dissociate rapidly upon ligation. Here, we report that binding of RXR tetramers to DNA containing two RXR response elements results in a dramatic DNA-looping. RXR can thus juxtapose distant DNA sequences, enabling transcriptional regulation by far-upstream factors. We show that RXR functions as a DNA architectural factor and that, while this activity is regulated by 9-cis-retinoic acid, it is distinct from and independent of the receptor's intrinsic transcriptional activity. The data establish RXR as the first identified architectural factor whose activity is regulated by a small ligand, and demonstrate a novel mechanism of transcriptional regulation by retinoids.

Idioma originalEnglish (US)
Páginas (desde-hasta)327-338
Número de páginas12
PublicaciónJournal of Molecular Biology
Volumen343
N.º2
DOI
EstadoPublished - oct. 15 2004

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Molecular Biology

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