Dissociation and flow cytometric isolation of murine intestinal epithelial cells for multi-omic profiling

Yong Ge; Mojgan Zadeh; Mansour Mohamadzadeh

doi:10.1016/j.xpro.2022.101936

Dissociation and flow cytometric isolation of murine intestinal epithelial cells for multi-omic profiling

Yong Ge, Mojgan Zadeh, Mansour Mohamadzadeh

Resultado de la investigación: Article › revisión exhaustiva

Resumen

Intestinal epithelium is composed of several cell types, which can be dissociated but difficult to maintain high cell viability due to anoikis. Herein, we describe a step-by-step protocol for the isolation of highly viable intestinal epithelial cells using ethylenediaminetetraacetate acid and TrypLE Express, which can subsequently be employed for multi-omic analyses, including single-cell RNA sequencing. For complete details on the use and execution of this protocol, please refer to Ge et al. (2022).¹

Idioma original	English (US)
Número de artículo	101936
Publicación	STAR Protocols
Volumen	4
N.º	1
DOI	https://doi.org/10.1016/j.xpro.2022.101936
Estado	Published - mar 17 2023

ASJC Scopus subject areas

Neuroscience(all)
Biochemistry, Genetics and Molecular Biology(all)
Immunology and Microbiology(all)

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10.1016/j.xpro.2022.101936

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title = "Dissociation and flow cytometric isolation of murine intestinal epithelial cells for multi-omic profiling",

abstract = "Intestinal epithelium is composed of several cell types, which can be dissociated but difficult to maintain high cell viability due to anoikis. Herein, we describe a step-by-step protocol for the isolation of highly viable intestinal epithelial cells using ethylenediaminetetraacetate acid and TrypLE Express, which can subsequently be employed for multi-omic analyses, including single-cell RNA sequencing. For complete details on the use and execution of this protocol, please refer to Ge et al. (2022).1",

keywords = "Cell isolation, Flow Cytometry/Mass Cytometry, Sequencing, Single Cell",

author = "Yong Ge and Mojgan Zadeh and Mansour Mohamadzadeh",

note = "Funding Information: This research was supported by NIH R01 DK109560 , NIH R01 DK109560C , and NIH R01AI154630-01 to M.M. Publisher Copyright: {\textcopyright} 2022 The Authors",

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AU - Ge, Yong

AU - Zadeh, Mojgan

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PY - 2023/3/17

Y1 - 2023/3/17

N2 - Intestinal epithelium is composed of several cell types, which can be dissociated but difficult to maintain high cell viability due to anoikis. Herein, we describe a step-by-step protocol for the isolation of highly viable intestinal epithelial cells using ethylenediaminetetraacetate acid and TrypLE Express, which can subsequently be employed for multi-omic analyses, including single-cell RNA sequencing. For complete details on the use and execution of this protocol, please refer to Ge et al. (2022).1

AB - Intestinal epithelium is composed of several cell types, which can be dissociated but difficult to maintain high cell viability due to anoikis. Herein, we describe a step-by-step protocol for the isolation of highly viable intestinal epithelial cells using ethylenediaminetetraacetate acid and TrypLE Express, which can subsequently be employed for multi-omic analyses, including single-cell RNA sequencing. For complete details on the use and execution of this protocol, please refer to Ge et al. (2022).1

KW - Cell isolation

KW - Flow Cytometry/Mass Cytometry

KW - Sequencing

KW - Single Cell

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Dissociation and flow cytometric isolation of murine intestinal epithelial cells for multi-omic profiling

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