Dissociation and flow cytometric isolation of murine intestinal epithelial cells for multi-omic profiling

Yong Ge, Mojgan Zadeh, Mansour Mohamadzadeh

Producción científica: Articlerevisión exhaustiva

1 Cita (Scopus)

Resumen

Intestinal epithelium is composed of several cell types, which can be dissociated but difficult to maintain high cell viability due to anoikis. Herein, we describe a step-by-step protocol for the isolation of highly viable intestinal epithelial cells using ethylenediaminetetraacetate acid and TrypLE Express, which can subsequently be employed for multi-omic analyses, including single-cell RNA sequencing. For complete details on the use and execution of this protocol, please refer to Ge et al. (2022).1

Idioma originalEnglish (US)
Número de artículo101936
PublicaciónSTAR Protocols
Volumen4
N.º1
DOI
EstadoPublished - mar 17 2023

ASJC Scopus subject areas

  • General Immunology and Microbiology
  • General Biochemistry, Genetics and Molecular Biology
  • General Neuroscience

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