Differential calmodulin-modulatory and electron transfer properties of neuronal nitric oxide synthase mu compared to the alpha variant

Satya P. Panda, Wenbing Li, Priya Venkatakrishnan, Li Chen, Andrei V. Astashkin, Bettie Sue S. Masters, Changjian Feng, Linda J. Roman

Resultado de la investigación: Articlerevisión exhaustiva

16 Citas (Scopus)

Resumen

Neuronal nitric oxide synthase μ (nNOSμ) contains 34 additional residues in an autoregulatory element compared to nNOSα. Cytochrome c and flavin reductions in the absence of calmodulin (CaM) were faster in nNOSμ than nNOSα, while rates in the presence of CaM were smaller. The magnitude of stimulation by CaM is thus notably lower in nNOSμ. No difference in NO production was observed, while electron transfer between the FMN and heme moieties and formation of an inhibitory ferrous-nitrosyl complex were slower in nNOSμ. Thus, the insert affects electron transfer rates, modulation of electron flow by CaM, and heme-nitrosyl complex formation.

Idioma originalEnglish (US)
Páginas (desde-hasta)3973-3978
Número de páginas6
PublicaciónFEBS Letters
Volumen587
N.º24
DOI
EstadoPublished - dic. 11 2013

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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