Dietary Ca is an important modulator of blood pressure in the spontaneously hypertensive rat (SHR). Since the kidney plays a key role in the pathogenesis of hypertension, the purpose of this study was to determine the potential renal mechanisms of the blood pressure-lowering effect of increasing dietary Ca content. In 21-day-old SHR fed 0.1 vs. 3.6% Ca diet for 14 days, increasing dietary Ca had no significant effects on basal [704 ± 50 in 0.1% Ca vs. 784 ± 61 ng angiotensin I (ANG I)·mg-1·h-1 in 3.6% Ca, P = not significant (NS)], isoproterenol-stimulated (1,057 ± 52 in 0.1% Ca vs. 1,104 ± 59 ng ANG I·mg-1·h-1 in 3.6% Ca, P = NS), or angiotensin II (ANG II)-inhibited (370 ± 50 in 0.1% Ca vs. 411 ± 39 ng ANG I·mg-1·h-1 in 3.6% Ca, P = NS) renal superficial cortical slice renin release. In contrast, in apical brush-border membrane (BBM) vesicles isolated from the superficial cortex, increasing dietary Ca caused a significant decrease in ANG II binding, which was mediated by a decrease in the number of binding sites (B(max), 376 ± 14 in 0.1% Ca vs. 234 ± 6 fmol ANG II/mg BBM protein in 3.6% Ca, P < 0.01), and no change in the affinity [dissociation constant (K(d)), 17.8 ± 1.4 in 0.1% Ca vs. 13.4 ± 2.8 nM ANG II in 3.6% Ca, P = NS]. In addition, increasing dietary Ca caused a significant decrease in BBM vesicle Na+-H+ antiport, which was mediated by a decrease in the maximal activity (V(max)) (13.53 ± 0.17 in 0.1% Ca vs. 11.35 ± 0.21 nmol Na·5 s-1·mg BBM protein-1 in 3.6% Ca, P < 0.05) and no change in the affinity [Michaelis constant (K(m)), 8.65 ± 0.41 in 0.1% Ca vs. 8.64 ± 0.35 mM Na in 3.6% Ca, P = NS]. Our study therefore indicates that increasing dietary Ca causes decreases in renal BBM ANG II binding sites and Na+-H+ antiport activity without changing intrarenal renin secretion. These intrarenal effects of dietary Ca on ANG II binding and Na+-H+ antiport may be important mechanisms of the blood pressure-modulating effect of dietary Ca in the SHR.
|Idioma original||English (US)|
|Publicación||American Journal of Physiology - Renal Fluid and Electrolyte Physiology|
|Estado||Published - ene 1 1991|
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