@article{160e247d9e0c459189c546d11a24c9c3,
title = "Dichotomous regulation of group 3 innate lymphoid cells by nongastric Helicobacter species",
abstract = "Intestinal innate lymphoid cells (ILCs) contribute to the protective immunity and homeostasis of the gut, and the microbiota are critically involved in shaping ILC function. However, the role of the gut microbiota in regulating ILC development and maintenance still remains elusive. Here, we identified opposing effects on ILCs by two Helicobacter species, Helicobacter apodemus and Helicobacter typhlonius, isolated from immunocompromised mice. We demonstrated that the introduction of both Helicobacter species activated ILCs and induced gut inflammation; however, these Helicobacter species negatively regulated RORγt+ group 3 ILCs (ILC3s), especially T-bet+ ILC3s, and diminished their proliferative capacity. Thus, these findings underscore a previously unknown dichotomous regulation of ILC3s by Helicobacter species, and may serve as a model for further investigations to elucidate the host–microbe interactions that critically sustain the maintenance of intestinal ILC3s.",
keywords = "Helicobacter, Host–microbiome, ILC, Inflammation",
author = "Bostick, {John W.} and Yetao Wang and Zeli Shen and Yong Ge and Jeffrey Brown and Chen, {Zong ming E.} and Mansour Mohamadzadeh and Fox, {James G.} and Liang Zhou",
note = "Funding Information: We thank the entire L.Z. laboratory for help and suggestions. We thank Jacob Weiderman for assistance with the preparation and performance of 16S rRNA gene sequencing. We thank the Genomics Facility (The University of Chicago) for sequencing services and assistance. The work was supported by the NIH (AI132391 and DK105562 to L.Z.). L.Z. is a Pew Scholar in Biomedical Sciences, supported by the Pew Charitable Trusts, and an Investigator in the Pathogenesis of Infectious Disease, supported by the Burroughs Wellcome Fund. J.W.B. is partially supported by a supplement to DK105562. This work was made possible in part by NIH Instrumentation Grant 1S10 OD021676-01. Funding Information: ACKNOWLEDGMENTS. We thank the entire L.Z. laboratory for help and suggestions. We thank Jacob Weiderman for assistance with the preparation and performance of 16S rRNA gene sequencing. We thank the Genomics Facility (The University of Chicago) for sequencing services and assistance. The work was supported by the NIH (AI132391 and DK105562 to L.Z.). L.Z. is a Pew Scholar in Biomedical Sciences, supported by the Pew Charitable Trusts, and an Investigator in the Pathogenesis of Infectious Disease, supported by the Burroughs Wellcome Fund. J.W.B. is partially supported by a supplement to DK105562. This work was made possible in part by NIH Instrumentation Grant 1S10 OD021676-01. Publisher Copyright: {\textcopyright} 2019 National Academy of Sciences. All rights reserved.",
year = "2019",
month = dec,
day = "3",
doi = "10.1073/pnas.1908128116",
language = "English (US)",
volume = "116",
pages = "24760--24769",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
publisher = "National Academy of Sciences",
number = "49",
}