Derivatives of [³H]serotonin in organ cultures of hamster pineal gland

The purpose of this study was to determine the viability of the hamster pineal gland in organ culture and to test the effect of norepinephrine (NE) on [³H]serotonin derivatives. In this study, elevated levels of melatonin (7-fold, p<.05), 5-hydroxytrytophol (5-fold, p<.001), 5-methoxytryptophol (1.78-fold, p<.05), and depressed levels of 5-hydroxyindoleacetic acid (3.8-fold, p<.02) and methoxyindoleacetic acid (1.78-fold, p<.05) were detected in the glands following the addition of NE to the medium. In a separate experiment, melatonin concentration in the media was also periodically measured by radioimmunoassay to determine the viability of the organ culture over a four-day period. The melatonin level on day 2 (2321 ± 106 pg/gland) was significantly higher (p<0.01) than on day 3 (1542 ± 86 pg/gland) or day 4 (805 ± 39 pg/gland). The results of these experiments verify the viability of the hamster pineal organ culture and show that the gland responds to NE in vitro.