TY - JOUR
T1 - Concatemers in a rapidly sedimenting, replicating bacteriophage T7 DNA
AU - Serwer, Philip
AU - Greenhaw, Gisele A.
AU - Allen, Jerry L.
N1 - Funding Information:
For assistanced uringthe earlier stageso f this work, we thank Nancy L. Smith. For assistancew ith electron microscopyw e thank Shirly J. Hayes. For tech- nical assistance,w e thank Elena T. Moreno. For secretarial assistance,w e thank Joanne Williams and Donna Scoggins.F or a generousg ift of unglucosylated T4 DNA, we thank Dr. J. W. Wiberg. For advicec on-cerning the radiolabeling of T7 DNA, we thank Dr. Donna L. Montgomery.S upportw as receivedf rom the National Institutes of Health (Grant GM-24365) and from the Robert A. Welch Foundation (Grant AQ-
PY - 1982/12
Y1 - 1982/12
N2 - In previous studies, replicating bacteriophage T7 DNA was isolated as a rapidly sedimenting, multigenome-sized complex (100 S+ DNA), consisting of double-stranded DNA with some single-stranded regions. In the present study, 100 S+ DNA was digested with nuclease S1 and the products were fractionated by velocity sedimentation and analyzed by electron microscopy and digestion with restriction enzymes, followed by agarose gel electrophoresis. Some of the fragments released from 100 S+ DNA by nuclease S1 are linear, heterogeneous in length, and one to five times as long as mature T7 DNA. Uniformlength DNA's one, two, and four times the length of mature T7 DNA were also present in sufficiently large amounts to form visible bands during velocity sedimentation.
AB - In previous studies, replicating bacteriophage T7 DNA was isolated as a rapidly sedimenting, multigenome-sized complex (100 S+ DNA), consisting of double-stranded DNA with some single-stranded regions. In the present study, 100 S+ DNA was digested with nuclease S1 and the products were fractionated by velocity sedimentation and analyzed by electron microscopy and digestion with restriction enzymes, followed by agarose gel electrophoresis. Some of the fragments released from 100 S+ DNA by nuclease S1 are linear, heterogeneous in length, and one to five times as long as mature T7 DNA. Uniformlength DNA's one, two, and four times the length of mature T7 DNA were also present in sufficiently large amounts to form visible bands during velocity sedimentation.
UR - https://www.scopus.com/pages/publications/0020458551
UR - https://www.scopus.com/pages/publications/0020458551#tab=citedBy
U2 - 10.1016/0042-6822(82)90283-5
DO - 10.1016/0042-6822(82)90283-5
M3 - Article
C2 - 6294987
AN - SCOPUS:0020458551
SN - 0042-6822
VL - 123
SP - 474
EP - 479
JO - Virology
JF - Virology
IS - 2
ER -