@article{d53cfaca68504443846b02a68c2ccfce,
title = "Colony stimulating factor-1 producing endothelial cells and mesenchymal stromal cells maintain monocytes within a perivascular bone marrow niche",
abstract = "Macrophage colony stimulating factor-1 (CSF-1) plays a critical role in maintaining myeloid lineage cells. However, congenital global deficiency of CSF-1 (Csf1op/op) causes severe musculoskeletal defects that may indirectly affect hematopoiesis. Indeed, we show here that osteolineage-derived Csf1 prevented developmental abnormalities but had no effect on monopoiesis in adulthood. However, ubiquitous deletion of Csf1 conditionally in adulthood decreased monocyte survival, differentiation, and migration, independent of its effects on bone development. Bone histology revealed that monocytes reside near sinusoidal endothelial cells (ECs) and leptin receptor (Lepr)-expressing perivascular mesenchymal stromal cells (MSCs). Targeted deletion of Csf1 from sinusoidal ECs selectively reduced Ly6C− monocytes, whereas combined depletion of Csf1 from ECs and MSCs further decreased Ly6Chi cells. Moreover, EC-derived CSF-1 facilitated recovery of Ly6C− monocytes and protected mice from weight loss following induction of polymicrobial sepsis. Thus, monocytes are supported by distinct cellular sources of CSF-1 within a perivascular BM niche.",
keywords = "LEPR, LPS, Ly6Chigh monocytes, Ly6Cnegative monocytes, MCSF1, bone development, bone marrow niche, endothelial cell, hematopoeisis, lipopolysaccharide, macrophage, macrophage colony stimulating factor, mesenchymal stromal cell, monocyte, monopoiesis, osteoblast, osteocyte, osteopetrosis, perivascular niche, perivascular stromal cell, sepsis, sinusoid",
author = "Takuo Emoto and Jessie Lu and Tharini Sivasubramaniyam and Hassaan Maan and Khan, {Aniqa B.} and Abow, {Amina A.} and Schroer, {Stephanie A.} and Hyduk, {Sharon J.} and Althagafi, {Marwan G.} and McKee, {Trevor D.} and Fred Fu and Shiva Shabro and Antigona Ulndreaj and Felix Chiu and Elvira Paneda and Shaun Pacheco and Tao Wang and Angela Li and Jiang, {Jean X.} and Peter Libby and Mansoor Husain and Bo Wang and Rubin, {Barry B.} and Cybulsky, {Myron I.} and Robbins, {Clinton S.}",
note = "Funding Information: We would like to acknowledge the administrative assistance of Bani Bali. This work was supported by CIHR awards ( 376108 , 426699 , 463387 ) and the Peter Munk Chair in Aortic Disease Research (C.S.R.). This research is part of the University of Toronto{\textquoteright}s Medicine by Design initiative, which receives funding from the Canada First Research Excellence Fund (CFREF). T.E. and T.S. were supported by post-doctoral research scholarships awarded by the Toronto General Research Institute . T.E. was also supported by a scholarship from SENSHIN Medical Research Foundation . J.L. was supported by an Ontario Graduate Scholarship . J.X.J. was supported by US National Institutes of Health grant AG045040 and Welch Foundation grant AQ-1507 . Publisher Copyright: {\textcopyright} 2022 Elsevier Inc.",
year = "2022",
month = may,
day = "10",
doi = "10.1016/j.immuni.2022.04.005",
language = "English (US)",
volume = "55",
pages = "862--878.e8",
journal = "Immunity",
issn = "1074-7613",
publisher = "Cell Press",
number = "5",
}