Resumen
A method for extracting from biological tissues vincristine, vinblastine, and their metabolites and analysis by high-performance liquid chromatography has been developed. After excision tissues are rapidly frozen in liquid nitrogen (<10 s) and powders are made under liquid N2. Extraction of blood, plasma, or tissue powders was achieved using ethanol (95%) acidified to pH 4.9 with acetic acid. Extracts were analyzed using reverse-phase chromatography capable of separating Vinca alkaloids with substitutions on the vindoline or catharanthine moiety. This technique has been used to elucidate the metabolism of [3H]vincristine and [3H]vinblastine in vivo and in vitro.
Idioma original | English (US) |
---|---|
Páginas (desde-hasta) | 450-454 |
Número de páginas | 5 |
Publicación | Analytical Biochemistry |
Volumen | 134 |
N.º | 2 |
DOI | |
Estado | Published - oct 15 1983 |
Publicado de forma externa | Sí |
ASJC Scopus subject areas
- Molecular Biology
- Biophysics
- Biochemistry
- Cell Biology