Cholesterol immobilization via ether-linked sepharose gels

Rajiv K. Satsangi; Glen E. Mott

doi:10.1039/an9921700953

Cholesterol immobilization via ether-linked sepharose gels

Rajiv K. Satsangi, Glen E. Mott

Resultado de la investigación: Article › revisión exhaustiva

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Resumen

Cholesterol has been immobilized on Sepharose-6B via oxyether linkages to the 3- or 25-position. The 3- or 25-hydroxysterol methanesulfonates were coupled with epoxy-Sepharose-6B at 80 °C for 24 h. Approximately 2% of the ligand was incorporated into the gel. These types of affinity columns may be useful in purifying proteins that specifically bind or metabolize cholesterol.

Idioma original	English (US)
Páginas (desde-hasta)	953-957
Número de páginas	5
Publicación	The Analyst
Volumen	117
N.º	6
DOI	https://doi.org/10.1039/an9921700953
Estado	Published - 1992
Publicado de forma externa	Sí

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Environmental Chemistry
Spectroscopy
Electrochemistry

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title = "Cholesterol immobilization via ether-linked sepharose gels",

abstract = "Cholesterol has been immobilized on Sepharose-6B via oxyether linkages to the 3- or 25-position. The 3- or 25-hydroxysterol methanesulfonates were coupled with epoxy-Sepharose-6B at 80 °C for 24 h. Approximately 2% of the ligand was incorporated into the gel. These types of affinity columns may be useful in purifying proteins that specifically bind or metabolize cholesterol.",

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author = "Satsangi, {Rajiv K.} and Mott, {Glen E.}",

year = "1992",

doi = "10.1039/an9921700953",

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AU - Mott, Glen E.

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KW - 3β-tert-butyldimethylsilyloxychotest- 5-en-25-one

KW - Cholesterol linked affinity gels

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Cholesterol immobilization via ether-linked sepharose gels

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