Cholesterol immobilization via ether-linked sepharose gels

Rajiv K. Satsangi, Glen E. Mott

Resultado de la investigación: Articlerevisión exhaustiva

1 Cita (Scopus)

Resumen

Cholesterol has been immobilized on Sepharose-6B via oxyether linkages to the 3- or 25-position. The 3- or 25-hydroxysterol methanesulfonates were coupled with epoxy-Sepharose-6B at 80 °C for 24 h. Approximately 2% of the ligand was incorporated into the gel. These types of affinity columns may be useful in purifying proteins that specifically bind or metabolize cholesterol.

Idioma originalEnglish (US)
Páginas (desde-hasta)953-957
Número de páginas5
PublicaciónThe Analyst
Volumen117
N.º6
DOI
EstadoPublished - 1992
Publicado de forma externa

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

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