@inbook{837533767af54f66b281f566f2838614,
title = "Characterizing the Electron Transport Chain: Structural Approach",
abstract = "The mitochondrial respiratory chain which carries out the oxidative phosphorylation (OXPHOS) consists of five multi-subunit protein complexes. Emerging evidences suggest that the supercomplexes which further consist of multiple respiratory complexes play important role in regulating OXPHOS function. Dysfunction of the respiratory chain and its regulation has been implicated in various human diseases including neurodegenerative diseases and muscular disorders. Many mouse models have been established which exhibit mitochondrial defects in brain and muscles. Protocols presented here aim to help to analyze the structures of mitochondrial respiratory chain which include the preparation of the tissue samples, isolation of mitochondrial membrane proteins, and analysis of their respiratory complexes by Blue Native Polyacrylamide Gel Electrophoresis (BN-PAGE) in particular.",
keywords = "Assembly, Blue Native Gel, Brain, Muscle, Respiratory complex",
author = "Ting Liang and Janice Deng and Bijaya Nayak and Xin Zou and Yuji Ikeno and Yidong Bai",
note = "Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2309-1_7",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "107--115",
booktitle = "Methods in Molecular Biology",
address = "United States",
}