Characterization of a unique ClpB protein of Mycoplasma pneumoniae and its impact on growth

T. R. Kannan, Oxana Musatovova, Pramod Gowda, Joel B. Baseman

Producción científica: Articlerevisión exhaustiva

32 Citas (Scopus)


Mycoplasma pneumoniae accounts for 20 to 30% of all community-acquired pneumonia and has been associated with other airway pathologies, including asthma, and a range of extrapulmonary manifestations. Although the entire genomic sequence of M. pneumoniae has been completed, the functions of many of these genes in mycoplasma physiology are unknown. In this study, we focused on clpB, a well-known heat shock gene in other bacteria, to examine its role in mycoplasma growth. Transcriptional and translational analyses of heat shock in M. pneumoniae indicated that clpB is significantly upregulated, reinforcing its status as a critical responder to heat stress. Interestingly, M. pneumoniae ClpB does not use dual translational start points for ClpB synthesis, like other ClpB-characterized bacteria. Biochemical characterization of purified M. pneumoniae recombinant ClpB revealed casein- and lysine-independent ATPase activity and DnaK-DnaJ-GrpEdependent chaperone activity. An M. pneumoniae mini-Tn4001-integrated, clpB-null mutant was impaired in its ability to replicate under permissive growth conditions, demonstrating the growth-promoting status of ClpB.

Idioma originalEnglish (US)
Páginas (desde-hasta)5082-5092
Número de páginas11
PublicaciónInfection and immunity
EstadoPublished - nov 2008

ASJC Scopus subject areas

  • Infectious Diseases
  • Parasitology
  • Microbiology
  • Immunology


Profundice en los temas de investigación de 'Characterization of a unique ClpB protein of Mycoplasma pneumoniae and its impact on growth'. En conjunto forman una huella única.

Citar esto