Resumen
The action of endogenous membrane proteinases in membranes isolated from human red cells of various ages was assayed by three groups of methods: (1) determination of the amount of protein fragments released to the acid-soluble fraction; (2) monitoring of changes in ESR spectra of maleimide spin-labeled erythrocyte membranes; (3) electrophoretic methods; a two-dimensional analysis and analysis of the activity inside SDS-PAGE gels. For all the methods the effects of proteinase action were highest in ghosts isolated from the erythrocytes of middle age.
Idioma original | English (US) |
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Páginas (desde-hasta) | 173-177 |
Número de páginas | 5 |
Publicación | BBA - Biomembranes |
Volumen | 981 |
N.º | 2 |
DOI | |
Estado | Published - jun 6 1989 |
Publicado de forma externa | Sí |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Cell Biology