TY - JOUR
T1 - Changes in membrane potential of intact adipocytes measured with fluorescent dyes
AU - Bailey, Frederick
AU - Hill, Nathalie
AU - Malinski, Tadeusz
AU - Kiechle, Frederick
N1 - Funding Information:
This research was supported by a grant from William Beaumont Research Institute.
PY - 1989/6
Y1 - 1989/6
N2 - The change in transmembrane potential of rat adipocytes was measured using the fluorescent probes 3,3′-diethylthiadicarbocyanine iodide, [di-SC2(5)], and 3,3′-dipropylthiadicarbocyanine iodide, [di-SC3(5)]. The fluorimetric technique was calibrated by altering the potassium ion concentration while keeping the sum of potassium and sodium ions at a constant concentration of 153 mM. A 3% change in relative fluorescence was equivalent to a membrane potential change of 1 mV. This method was used to measure changes in transmembrane potential by insulin and two insulin-mimetic agents: wheat germ agglutinin and ethanolamine.
AB - The change in transmembrane potential of rat adipocytes was measured using the fluorescent probes 3,3′-diethylthiadicarbocyanine iodide, [di-SC2(5)], and 3,3′-dipropylthiadicarbocyanine iodide, [di-SC3(5)]. The fluorimetric technique was calibrated by altering the potassium ion concentration while keeping the sum of potassium and sodium ions at a constant concentration of 153 mM. A 3% change in relative fluorescence was equivalent to a membrane potential change of 1 mV. This method was used to measure changes in transmembrane potential by insulin and two insulin-mimetic agents: wheat germ agglutinin and ethanolamine.
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U2 - 10.1016/0302-4598(89)85011-1
DO - 10.1016/0302-4598(89)85011-1
M3 - Article
AN - SCOPUS:38249022687
SN - 0302-4598
VL - 21
SP - 333
EP - 342
JO - Bioelectrochemistry and Bioenergetics
JF - Bioelectrochemistry and Bioenergetics
IS - 3
ER -