Bradykinin and inflammatory pain

The pro-inflammatory pharmacology of bradykinin has been well established. However, knowledge of the actual tissue concentrations and pharmacological manipulation of immunoreactive bradykinin in clinical and animal models of inflammation remain relatively sparse. We have developed a microdialysis method to implant probes into the inflamed tissue compartment in order to collect dialysate continuously in patients following the surgical removal of impacted third molars. Dialysate samples can be analyzed for levels of inflammatory mediators (eg., bradykinin, prostaglandins, etc) and drugs in order to determine the time-response curves for local release of substances in inflamed tissue.