Block of granulocytic differentiation of 32Dcl3 cells by AML1/ETO(MTG8) but not by highly expressed Bcl-2

Hidetsugu Kohzaki, Kosei Ito, Gang Huang, Hee Jun Wee, Yota Murakami, Yoshiaki Ito

Resultado de la investigación: Articlerevisión exhaustiva

36 Citas (Scopus)

Resumen

The chimeric gene, AML1/ETO (MTG8), generated in t(8;21) acute myeloid leukemia enhances the expression of Bcl-2. To evaluate whether this enhancement is the primary role of AML1/ETO in leukemogenesis, effects of over-expression of Bcl-2 in the murine myeloid precursor cell line, 32Dcl3, were examined. When 32Dcl3 cells expressing exogenous Bcl-2 were induced to differentiate, the onset of morphological differentiation was delayed. However, even the cells expressing very high levels of exogenous Bcl-2 eventually underwent differentiation without a significant decrease in the synthesis of Bcl-2. On the contrary, 32Dcl3 cells stably expressing AML1/ETO were completely resistant to differentiation and continued to grow in the presence of G-CSF. These results are consistent with the interpretation that stimulation of Bcl-2 expression is not the primary target of AML1/ETO.

Idioma originalEnglish (US)
Páginas (desde-hasta)4055-4062
Número de páginas8
PublicaciónOncogene
Volumen18
N.º28
DOI
EstadoPublished - jul. 15 1999
Publicado de forma externa

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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