Asynchronous replication of imprinted genes is established in the gametes and maintained during development

Itamar Simon, Toyoaki Tenzen, Benjamin E. Reubinoff, Dahlia Hillman, John R. McCarrey, Howard Cedar

Producción científica: Articlerevisión exhaustiva

143 Citas (Scopus)

Resumen

Genomic imprinting is characterized by allele-specific expression of multiple genes within large chromosomal domains that undergo DNA replication asynchronously during S phase. Here we show, using both fluorescence in situ hybridization analysis and S-phase fractionation techniques, that differential replication timing is associated with imprinted genes in a variety of cell types, and is already present in the pre-implantation embryo soon after fertilization. This pattern is erased before meiosis in the germ line, and parent-specific replication timing is then reset in late gametogenesis in both the male and female. Thus, asynchronous replication timing is established in the gametes and maintained throughout development, indicating that it may function as a primary epigenetic marker for distinguishing between the parental alleles.

Idioma originalEnglish (US)
Páginas (desde-hasta)929-932
Número de páginas4
PublicaciónNature
Volumen401
N.º6756
DOI
EstadoPublished - oct 28 1999
Publicado de forma externa

ASJC Scopus subject areas

  • General

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