Assignment of the human peroxisomal branched-chain Acyl-CoA oxidase gene to chromosome 3p21.1-p14.2 by rodent/human somatic cell hybridization

PCR and rodent/human somatic cell hybrids were used to localize the human peroxisomal branched chain acyl-CoA oxidase gene. Oligonucleotide primers were chosen to specifically amplify human hBCox DNA. The amplified sequence contained two restriction enzyme sites which were used to verify the authenticity of the amplified DNA. Initially, the gene was localized to human chromosome 3 by screening genomic DNA from a hybrid mapping panel. Additional hybrids retaining well-characterized fragments of human chromosome 3 were screened to further localize the gene to 3p21.1-p14.2.