@inbook{660cb75e4c4e4159aacd7630211d94df,
title = "Assessment of airway hyperresponsiveness in murine tracheal rings",
abstract = "Isolated tracheal rings have often been used to directly measure the contractile output of airway smooth muscle (ASM). Here, we describe the method for excising murine tracheas, mounting tracheal rings in organ baths, and measuring the isometric forces generated by the ASM when stimulated by drug additions or electric field stimulation. The apparatus for the setup and the pathways responsible for stimulation are detailed. Examples of the responses and analyses of two types of ASM stimulation are illustrated: (1) the carbachol concentration-response curve and (2) the frequency-response curve elicited by electric field stimulation.",
keywords = "Airway smooth muscle, Concentration-response curves, Electric field stimulation, Hyperresponsiveness, Isometric force, Tracheal rings",
author = "Herlihy, {Jeremiah T.} and Iurii Semenov and Robert Brenner",
year = "2013",
doi = "10.1007/978-1-62703-496-8_20",
language = "English (US)",
isbn = "9781627034951",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "257--269",
booktitle = "Mouse Models of Allergic Disease",
address = "United States",
}