Approaches for Mapping and Analysis of R-loops

Pramiti Mukhopadhyay, Henry Miller, Aiola Stoja, Alexander J.R. Bishop

Producción científica: Articlerevisión exhaustiva

Resumen

R-loops are nucleic acid structures composed of a DNA:RNA hybrid with a displaced non-template single-stranded DNA. Current approaches to identify and map R-loop formation across the genome employ either an antibody targeted against R-loops (S9.6) or a catalytically inactivated form of RNase H1 (dRNH1), a nuclease that can bind and resolve DNA:RNA hybrids via RNA exonuclease activity. This overview article outlines several ways to map R-loops using either methodology, explaining the differences and similarities among the approaches. Bioinformatic analysis of R-loops involves several layers of quality control and processing before visualizing the data. This article provides resources and tools that can be used to accurately process R-loop mapping data and explains the advantages and disadvantages of the resources as compared to one another.

Idioma originalEnglish (US)
Número de artículoe1037
PublicaciónCurrent Protocols
Volumen4
N.º4
DOI
EstadoPublished - abr 2024

ASJC Scopus subject areas

  • General Neuroscience
  • General Biochemistry, Genetics and Molecular Biology
  • General Immunology and Microbiology
  • General Pharmacology, Toxicology and Pharmaceutics
  • Health Informatics
  • Medical Laboratory Technology

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