Apoptosis-induced by TRAIL and TNF-α in human multiple myeloma cells is not blocked by Bcl-2

Yair Gazitt, Paul Shaughnessy, Willi Montgomery

Resultado de la investigación: Articlerevisión exhaustiva

61 Citas (Scopus)

Resumen

TRAIL, the ligand for the newly discovered DR-4 and DR-5 receptor is a member of the tumour necrosis factor (TNF) family of death signal tranduction proteins with a mechanism of cell death, similar to the Fas and Fas ligand (Fas-L) system. Here, we provide first time evidence that TRAIL and TNF-α are potent inducers of apoptosis in multiple myeloma (MM) cell lines and freshly isolated myeloma cells. TRAIL effectively induced extensive apoptosis in 8226 and ARP-1 MM cells in a time- and dose-dependent manner reaching 80% within 48 h of treatment with a dose of 160 ng/ml. Bcl-2 transfected 8226 and ARP-1 cells were equally sensitive to apoptosis by TRAIL. Apoptosis with TNFα, reached > 60% within 48 h of treatment with a dose of 160 ng/ml. In addition to MM cell lines, freshly isolated, flow-sorted myeloma cells from 8 different MM patients expressing variable levels of bcl-2 mere equally sensitive to both TRAIL and TNF-α. We have previously shown that anti-Fas-induced apoptosis is not blocked by endogenous or ectopic bcl-2 in MM cell lines. Here we extend our observation with Fas to include TNF-α and TRAIL to the apoptotic signals that are not be blocked by bcl-2, in MM cells.

Idioma originalEnglish (US)
Páginas (desde-hasta)1010-1019
Número de páginas10
PublicaciónCytokine
Volumen11
N.º12
DOI
EstadoPublished - dic. 1999

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Hematology
  • Molecular Biology

Huella

Profundice en los temas de investigación de 'Apoptosis-induced by TRAIL and TNF-α in human multiple myeloma cells is not blocked by Bcl-2'. En conjunto forman una huella única.

Citar esto