Antibody responses to the chlamydial heat shock proteins hsp60 and hsp70 are H-2 linked

G. Zhong, R. C. Brunham

Producción científica: Articlerevisión exhaustiva

56 Citas (Scopus)

Resumen

The effects of both H-2 and non-H-2 genes on antibody responses to two Chlamydia trachomatis heat shock proteins (hsp60 and hsp70) were investigated. These chlamydial proteins are homologs of Escherichia coli GroEL (hsp60) and DnaK (hsp70) and are highly sequence conserved between bacterial and mammalian sources. Antibody responses among 17 different strains of mice immunized with C. trachomatis serovar B and serovar C elementary bodies were evaluated by immunoblot, radioimmunoprecipitation and enzyme-linked immunosorbent assay. Antibody responses to the two proteins displayed host genetic restriction. Of six distinctive H-2 haplotypes, only H-2(d) generated high antibody responses to hsp70. Five of the six H-2 haplotypes, i.e., H-2a, H-2(d), H-2(k), H-2(q), and H-2(s), produced high antibody responses to hsp60. Only the H-2b-bearing strain had low antibody responses to hsp60. By using congenic and H-2 recombinant strains, the genes responsible for regulating antibody responses to hsp70 and hsp60 were mapped to the K-IA region of the H-2 locus. In F1 hybrid crosses between high and low responders, high responses to hsp60 and hsp70 were dominant traits. Other genes outside the H-2 locus also influenced antibody responses to hsp60 and hsp70, since inbred strains of identical H-2 but different background genes displayed variable antibody responses to the proteins. The genetic control of murine immune responses to C. trachomatis hsp60, a putative chlamydial immunopathologic antigen, suggests that a similar genetic mechanism may also exist in humans, and this observation may help to explain the observed variability in the spectrum of chlamydial diseases seen in humans.

Idioma originalEnglish (US)
Páginas (desde-hasta)3143-3149
Número de páginas7
PublicaciónInfection and immunity
Volumen60
N.º8
EstadoPublished - 1992
Publicado de forma externa

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Huella

Profundice en los temas de investigación de 'Antibody responses to the chlamydial heat shock proteins hsp60 and hsp70 are H-2 linked'. En conjunto forman una huella única.

Citar esto