Analysis of mitochondrial electron transport complexes by MALDI-TOF mass spectrometry

Susan T. Weintraub; Andrej Musatov; Christopher A. Carroll; Neal C Robinson

Analysis of mitochondrial electron transport complexes by MALDI-TOF mass spectrometry

Susan T. Weintraub, Andrej Musatov, Christopher A. Carroll, Neal C Robinson

Department of Biochemistry & Structural Biology

Producción científica: Paper › revisión exhaustiva

1 Cita (Scopus)

Resumen

The mitochondrial electron transport complexes were analyzed using MALDI-TOF mass spectrometry (MS) molecular masses of the complexes, cytochrome c oxidase (CcO) and cytochrome bc ₁, were determined for the investigation on the oxidative damage to electron transport proteins. Prior to MS analysis, DM was exchanged for the detergent in the protein samples by dialysis. The addition of DM to solution of CcO and Cytochrome bc ₁ was found to aid in detection of all subunits of the complex proteins.

Idioma original	English (US)
Páginas	205-206
Número de páginas	2
Estado	Published - 2002
Evento	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duración: jun 2 2002 → jun 6 2002

Other

Other	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
País/Territorio	United States
Ciudad	Orlando, FL
Período	6/2/02 → 6/6/02

ASJC Scopus subject areas

Spectroscopy

Otros archivos y enlaces

Citar esto

@conference{5964794298a246cc8db88c84ca16c86d,

title = "Analysis of mitochondrial electron transport complexes by MALDI-TOF mass spectrometry",

abstract = "The mitochondrial electron transport complexes were analyzed using MALDI-TOF mass spectrometry (MS) molecular masses of the complexes, cytochrome c oxidase (CcO) and cytochrome bc 1, were determined for the investigation on the oxidative damage to electron transport proteins. Prior to MS analysis, DM was exchanged for the detergent in the protein samples by dialysis. The addition of DM to solution of CcO and Cytochrome bc 1 was found to aid in detection of all subunits of the complex proteins.",

author = "Weintraub, {Susan T.} and Andrej Musatov and Carroll, {Christopher A.} and Robinson, {Neal C}",

year = "2002",

language = "English (US)",

pages = "205--206",

note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

}

TY - CONF

T1 - Analysis of mitochondrial electron transport complexes by MALDI-TOF mass spectrometry

AU - Weintraub, Susan T.

AU - Musatov, Andrej

AU - Carroll, Christopher A.

AU - Robinson, Neal C

PY - 2002

Y1 - 2002

N2 - The mitochondrial electron transport complexes were analyzed using MALDI-TOF mass spectrometry (MS) molecular masses of the complexes, cytochrome c oxidase (CcO) and cytochrome bc 1, were determined for the investigation on the oxidative damage to electron transport proteins. Prior to MS analysis, DM was exchanged for the detergent in the protein samples by dialysis. The addition of DM to solution of CcO and Cytochrome bc 1 was found to aid in detection of all subunits of the complex proteins.

AB - The mitochondrial electron transport complexes were analyzed using MALDI-TOF mass spectrometry (MS) molecular masses of the complexes, cytochrome c oxidase (CcO) and cytochrome bc 1, were determined for the investigation on the oxidative damage to electron transport proteins. Prior to MS analysis, DM was exchanged for the detergent in the protein samples by dialysis. The addition of DM to solution of CcO and Cytochrome bc 1 was found to aid in detection of all subunits of the complex proteins.

UR - http://www.scopus.com/inward/record.url?scp=2442703197&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2442703197&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:2442703197

SP - 205

EP - 206

T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

Analysis of mitochondrial electron transport complexes by MALDI-TOF mass spectrometry

Resumen

Other

ASJC Scopus subject areas

Otros archivos y enlaces

Huella

Citar esto