An assembled complex IV maintains the stability and activity of complex I in mammalian mitochondria

Youfen Li, Marilena D'Aurelio, Jian Hong Deng, Jeong Soon Park, Giovanni Manfredi, Peiqing Hu, Jianxin Lu, Yidong Bai

Producción científica: Articlerevisión exhaustiva

111 Citas (Scopus)

Resumen

In the mammalian mitochondrial electron transfer system, the majority of electrons enter at complex I, go through complexes III and IV, and are finally delivered to oxygen. Previously we generated several mouse cell lines with suppressed expression of the nuclearly encoded subunit 4 of complex IV. This led to a loss of assembly of complex IV and its defective function. Interestingly, we found that the level of assembled complex I and its activity were also significantly reduced, whereas levels and activity of complex III were normal or up-regulated. The structural and functional dependence of complex I on complex IV was verified using a human cell line carrying a nonsense mutation in the mitochondrially encoded complex IV subunit 1 gene. Our work documents that, although there is no direct electron transfer between them, an assembled complex IV helps to maintain complex I in mammalian cells.

Idioma originalEnglish (US)
Páginas (desde-hasta)17557-17562
Número de páginas6
PublicaciónJournal of Biological Chemistry
Volumen282
N.º24
DOI
EstadoPublished - jun 15 2007

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry
  • Cell Biology

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