Agrobacterium tumefaciens-Mediated Transformation of Candida glabrata

Samantha D’spain, Pilar I. Andrade, Nohelli E. Brockman, Jianmin Fu, Brian L. Wickes

Producción científica: Articlerevisión exhaustiva

2 Citas (Scopus)

Resumen

The use of broad-spectrum antimycotic therapy, immunosuppressive therapy, and in-dwelling medical devices has contributed to the increased frequency of mucosal and systemic infections caused by Candida glabrata. A major concern for C. glabrata and other Candida spp. infections is the increase in drug resistance. To address these issues, additional molecular tools for the study of C. glabrata are needed. In this investigation, we developed an Agrobacterium tumefaciens transformation system for C. glabrata. A number of parameters were investigated to determine their effect on transformation frequency, and then an optimized protocol was developed. The optimal conditions for the transformation of C. glabrata were found to be an infection incubation temperature of 26 C, 0.2 mM acetosyringone in both induction media and co-culture media, 0.7% agar concentra-tion, and a multiplicity of infection of 50:1 A. tumefaciens to C. glabrata. Importantly, the frequency of multiple integrations was low (5%), demonstrating that A. tumefaciens generally integrates at single sites in C. glabrata, which is consistent with other fungal A. tumefaciens transformation systems. The development of this system in C. glabrata adds another tool for the molecular manipulation of this increasingly important fungal pathogen.

Idioma originalEnglish (US)
Número de artículo596
PublicaciónJournal of Fungi
Volumen8
N.º6
DOI
EstadoPublished - jun 2022
Publicado de forma externa

ASJC Scopus subject areas

  • Microbiology (medical)
  • Ecology, Evolution, Behavior and Systematics
  • Plant Science

Huella

Profundice en los temas de investigación de 'Agrobacterium tumefaciens-Mediated Transformation of Candida glabrata'. En conjunto forman una huella única.

Citar esto