Age-related base excision repair activity in mouse brain and liver nuclear extracts

Gabriel W. Intano, Eun Ju Cho, C. Alex McMahan, Christi A. Walter

Producción científica: Articlerevisión exhaustiva

79 Citas (Scopus)

Resumen

To assess DNA repair activity relative to age, in vitro base excision repair assays were performed using brain and liver nuclear extracts prepared from mice of various ages. An 85% decline in repair activity was observed in brain nuclear extracts and a 50% decrease in liver nuclear extracts prepared from old mice compared with 6-day-old mice. Brain nuclear extracts prepared from old mice showed a decreased abundance of DNA polymerase-β, but the addition of purified protein did not restore base excision repair activity. Abundances of other tested base excision repair proteins did not change relative to age. The conclusion is that, during aging, a decline in DNA repair could contribute to increased levels of DNA damage and mutagenesis.

Idioma originalEnglish (US)
Páginas (desde-hasta)205-211
Número de páginas7
PublicaciónJournals of Gerontology - Series A Biological Sciences and Medical Sciences
Volumen58
N.º3
DOI
EstadoPublished - mar 1 2003

ASJC Scopus subject areas

  • General Medicine

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