Adipokine expression is associated with adipocyte volume in baboons

M. E. Tejero; J. M. Proffitt; I. P. Rodríguez; G. Hubbard; Jeanne H. Freeland-Graves; Kyle W. Peebles; Shelley A. Cole; Anthony Comuzzie

doi:10.1016/j.cyto.2007.11.005

Adipokine expression is associated with adipocyte volume in baboons

M. E. Tejero, J. M. Proffitt, I. P. Rodríguez, G. Hubbard, Jeanne H. Freeland-Graves, Kyle W. Peebles, Shelley A. Cole, Anthony Comuzzie

Resultado de la investigación: Article › revisión exhaustiva

5 Citas (Scopus)

Resumen

Baboons show significant variation in body weight and composition, coupled with insulin resistance and phenotypes associated with the metabolic syndrome. An omental adipose tissue biopsy and a fasting blood sample were collected from 40 unrelated adult baboons from the colony at Southwest Foundation for Biomedical Research in San Antonio, TX. Serum was separated for analyses of circulating levels of glucose, insulin, adiponectin, resistin, interleukin 6 (IL-6) and monocyte chemoattractant protein-1 (MCP-1or CCL-2). Adipose tissue biopsies were analyzed for cell volume and number. Total RNA was isolated from adipose tissue and adiponectin, resistin, δ-resistin, MCP-1 and IL-6 mRNA abundance were measured using real time, quantitative RT-PCR. Partial correlation coefficients were calculated among adipokine expression, fat tissue cell volume, and circulating levels of proteins. Cell volume was significantly correlated with expression of MCP-1 (r = 0.44, p < 0.05) and IL-6 mRNA (r = 0.47, p < 0.01). A step wise regression analysis was conducted with adipose tissue cell volume as dependent variable. The model identified IL-6 mRNA levels in adipose tissue as the only predictor. These observations support the role of IL-6 as a possible paracrine regulator in adipose tissue.

Idioma original	English (US)
Páginas (desde-hasta)	150-154
Número de páginas	5
Publicación	Cytokine
Volumen	41
N.º	2
DOI	https://doi.org/10.1016/j.cyto.2007.11.005
Estado	Published - feb 2008
Publicado de forma externa	Sí

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Biochemistry
Molecular Biology
Hematology

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10.1016/j.cyto.2007.11.005

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title = "Adipokine expression is associated with adipocyte volume in baboons",

abstract = "Baboons show significant variation in body weight and composition, coupled with insulin resistance and phenotypes associated with the metabolic syndrome. An omental adipose tissue biopsy and a fasting blood sample were collected from 40 unrelated adult baboons from the colony at Southwest Foundation for Biomedical Research in San Antonio, TX. Serum was separated for analyses of circulating levels of glucose, insulin, adiponectin, resistin, interleukin 6 (IL-6) and monocyte chemoattractant protein-1 (MCP-1or CCL-2). Adipose tissue biopsies were analyzed for cell volume and number. Total RNA was isolated from adipose tissue and adiponectin, resistin, δ-resistin, MCP-1 and IL-6 mRNA abundance were measured using real time, quantitative RT-PCR. Partial correlation coefficients were calculated among adipokine expression, fat tissue cell volume, and circulating levels of proteins. Cell volume was significantly correlated with expression of MCP-1 (r = 0.44, p < 0.05) and IL-6 mRNA (r = 0.47, p < 0.01). A step wise regression analysis was conducted with adipose tissue cell volume as dependent variable. The model identified IL-6 mRNA levels in adipose tissue as the only predictor. These observations support the role of IL-6 as a possible paracrine regulator in adipose tissue.",

keywords = "Adipocyte, Adipokines, Animal model, IL-6, Inflammation, Interleukin-6",

author = "Tejero, {M. E.} and Proffitt, {J. M.} and Rodr{\'i}guez, {I. P.} and G. Hubbard and Freeland-Graves, {Jeanne H.} and Peebles, {Kyle W.} and Cole, {Shelley A.} and Anthony Comuzzie",

note = "Funding Information: This work was funded by NIH Grants HL28972 and 251RR013986. This investigation was conducted in a facility constructed with support from Research Facilities Improvement Program Grant No. C06 RR13556 from the National Center for Research Resources, NIH.",

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doi = "10.1016/j.cyto.2007.11.005",

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AU - Tejero, M. E.

AU - Proffitt, J. M.

AU - Rodríguez, I. P.

AU - Hubbard, G.

AU - Freeland-Graves, Jeanne H.

AU - Peebles, Kyle W.

AU - Cole, Shelley A.

AU - Comuzzie, Anthony

N1 - Funding Information: This work was funded by NIH Grants HL28972 and 251RR013986. This investigation was conducted in a facility constructed with support from Research Facilities Improvement Program Grant No. C06 RR13556 from the National Center for Research Resources, NIH.

PY - 2008/2

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N2 - Baboons show significant variation in body weight and composition, coupled with insulin resistance and phenotypes associated with the metabolic syndrome. An omental adipose tissue biopsy and a fasting blood sample were collected from 40 unrelated adult baboons from the colony at Southwest Foundation for Biomedical Research in San Antonio, TX. Serum was separated for analyses of circulating levels of glucose, insulin, adiponectin, resistin, interleukin 6 (IL-6) and monocyte chemoattractant protein-1 (MCP-1or CCL-2). Adipose tissue biopsies were analyzed for cell volume and number. Total RNA was isolated from adipose tissue and adiponectin, resistin, δ-resistin, MCP-1 and IL-6 mRNA abundance were measured using real time, quantitative RT-PCR. Partial correlation coefficients were calculated among adipokine expression, fat tissue cell volume, and circulating levels of proteins. Cell volume was significantly correlated with expression of MCP-1 (r = 0.44, p < 0.05) and IL-6 mRNA (r = 0.47, p < 0.01). A step wise regression analysis was conducted with adipose tissue cell volume as dependent variable. The model identified IL-6 mRNA levels in adipose tissue as the only predictor. These observations support the role of IL-6 as a possible paracrine regulator in adipose tissue.

AB - Baboons show significant variation in body weight and composition, coupled with insulin resistance and phenotypes associated with the metabolic syndrome. An omental adipose tissue biopsy and a fasting blood sample were collected from 40 unrelated adult baboons from the colony at Southwest Foundation for Biomedical Research in San Antonio, TX. Serum was separated for analyses of circulating levels of glucose, insulin, adiponectin, resistin, interleukin 6 (IL-6) and monocyte chemoattractant protein-1 (MCP-1or CCL-2). Adipose tissue biopsies were analyzed for cell volume and number. Total RNA was isolated from adipose tissue and adiponectin, resistin, δ-resistin, MCP-1 and IL-6 mRNA abundance were measured using real time, quantitative RT-PCR. Partial correlation coefficients were calculated among adipokine expression, fat tissue cell volume, and circulating levels of proteins. Cell volume was significantly correlated with expression of MCP-1 (r = 0.44, p < 0.05) and IL-6 mRNA (r = 0.47, p < 0.01). A step wise regression analysis was conducted with adipose tissue cell volume as dependent variable. The model identified IL-6 mRNA levels in adipose tissue as the only predictor. These observations support the role of IL-6 as a possible paracrine regulator in adipose tissue.

KW - Adipocyte

KW - Adipokines

KW - Animal model

KW - IL-6

KW - Inflammation

KW - Interleukin-6

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Adipokine expression is associated with adipocyte volume in baboons

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ASJC Scopus subject areas

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