@inbook{8ebcc1f28a1b4c7fbac531845018e668,
title = "Activity detection of GalNAc transferases by protein-based fluorescence sensors in vivo",
abstract = "Mucin-type O-glycosylation occurring in the Golgi apparatus is an important protein posttranslational modification initiated by up to 20 GalNAc-transferase isozymes with largely distinct substrate specificities. Regulation of this enzyme family affects a vast array of proteins transiting the secretory pathway and misregulation causes human diseases. Here we describe the use of protein-based fluorescence sensors that traffic in the secretory pathway to monitor GalNAc-transferase activity in living cells. The sensors can either be “pan” or isozyme specific.",
keywords = "Fluorescence-activating protein, Fluorescent biosensor, GalNAc transferase, O-glycosylation",
author = "Lina Song and Collin Bachert and Linstedt, {Adam D.}",
note = "Funding Information: This work was supported by NIH grant GM056779 to A.D.L. Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2016.",
year = "2016",
month = sep,
day = "1",
doi = "10.1007/978-1-4939-6463-5_10",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "123--131",
booktitle = "Methods in Molecular Biology",
address = "United States",
}