A simple method for the quantitation of isozyme patterns

Robert J. Klebe

Resultado de la investigación: Articlerevisión exhaustiva

41 Citas (Scopus)


Isozyme patterns may be analyzed quantitatively, without the use of a densitometer, by performing serial twofold dilutions of a sample to a visual end point. The specific activity (S) of a given dehydrogenase isozyme can be assessed in the presence of other isozymes catalyzing the same reaction, by (1) determining the isozyme titer (T) (defined as mg protein/ml in the last visible band) and (2) applying the formula S=K/T, where K is 1.6×10-3 units/ml in the last visible band. The units/ml (U) in the starting material can be calculated from the equation U=K (2)n-1, where n is the number of the slot producing the last visible band.

Idioma originalEnglish (US)
Páginas (desde-hasta)805-812
Número de páginas8
PublicaciónBiochemical Genetics
EstadoPublished - dic 1975
Publicado de forma externa

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Biochemistry
  • Molecular Biology
  • Genetics


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