@article{aa8761db51354ff68e09a0a0f96cc722,
title = "A reference tissue atlas for the human kidney",
abstract = "Kidney Precision Medicine Project (KPMP) is building a spatially specified human kidney tissue atlas in health and disease with single-cell resolution. Here, we describe the construction of an integrated reference map of cells, pathways, and genes using unaffected regions of nephrectomy tissues and undiseased human biopsies from 56 adult subjects. We use single-cell/nucleus transcriptomics, subsegmental laser microdissection transcriptomics and proteomics, near-single-cell proteomics, 3D and CODEX imaging, and spatial metabolomics to hierarchically identify genes, pathways, and cells. Integrated data from these different technologies coherently identify cell types/subtypes within different nephron segments and the interstitium. These profiles describe cell-level functional organization of the kidney following its physiological functions and link cell subtypes to genes, proteins, metabolites, and pathways. They further show that messenger RNA levels along the nephron are congruent with the subsegmental physiological activity. This reference atlas provides a framework for the classification of kidney disease when multiple molecular mechanisms underlie convergent clinical phenotypes.",
author = "{Kidney Precision Medicine Project} and Jens Hansen and Rachel Sealfon and Rajasree Menon and Eadon, {Michael T.} and Lake, {Blue B.} and Becky Steck and Kavya Anjani and Samir Parikh and Sigdel, {Tara K.} and Guanshi Zhang and Dusan Velickovic and Daria Barwinska and Theodore Alexandrov and Dejan Dobi and Priyanka Rashmi and Otto, {Edgar A.} and Miguel Rivera and Rose, {Michael P.} and Anderton, {Christopher R.} and Shapiro, {John P.} and Annapurna Pamreddy and Seth Winfree and Yuguang Xiong and Yongqun He and {de Boer}, {Ian H.} and Hodgin, {Jeffrey B.} and Laura Barisoni and Naik, {Abhijit S.} and Kumar Sharma and Sarwal, {Minnie M.} and Kun Zhang and Jonathan Himmelfarb and Brad Rovin and El-Achkar, {Tarek M.} and Zoltan Laszik and He, {John Cijiang} and Dagher, {Pierre C.} and {Todd Valerius}, M. and Sanjay Jain and Satlin, {Lisa M.} and Troyanskaya, {Olga G.} and Matthias Kretzler and Ravi Iyengar and Azeloglu, {Evren U.}",
note = "Funding Information: KPMP acknowledges all the participants, patients, and the scientific officers from the National Institute of Diabetes and Digestive and Kidney Diseases. KPMP was supported by NIH grants UH3 DK114923, UH3 DK114920, UH3 DK114933, UH3 DK114937, UH3 DK114907, and U2C DK114886. A complete list of all KPMP members can be found at kpmp.org. We thank J. Goldfarb for critically reading of the manuscript. Publisher Copyright: Copyright {\textcopyright} 2022 The Authors, some rights reserved;",
year = "2022",
month = jun,
doi = "10.1126/sciadv.abn4965",
language = "English (US)",
volume = "8",
journal = "Science advances",
issn = "2375-2548",
publisher = "American Association for the Advancement of Science",
number = "23",
}