A next-generation proteome array for Schistosoma mansoni

Rafael Ramiro de Assis; Fernanda Ludolf; Rie Nakajima; Al Jasinskas; Guilherme C. Oliveira; Philip L. Felgner; Soraya T. Gaze; Alex Loukas; Philip T. LoVerde; Jeffrey M. Bethony; Rodrigo Correa-Oliveira; Carlos E. Calzavara-Silva

doi:10.1016/j.ijpara.2016.04.001

A next-generation proteome array for Schistosoma mansoni

Rafael Ramiro de Assis, Fernanda Ludolf, Rie Nakajima, Al Jasinskas, Guilherme C. Oliveira, Philip L. Felgner, Soraya T. Gaze, Alex Loukas, Philip T. LoVerde, Jeffrey M. Bethony, Rodrigo Correa-Oliveira, Carlos E. Calzavara-Silva

Department of Biochemistry & Structural Biology

Resultado de la investigación: Article › revisión exhaustiva

17 Citas (Scopus)

Resumen

A proteome microarray consisting of 992 Schistosoma mansoni proteins was produced and screened with sera to determine antibody signatures indicative of the clinical stages of schistosomiasis and the identification of subunit vaccine candidates. Herein, we describe the methods used to derive the gene list for this array (representing approximately 10% of the predicted S. mansoni proteome). We also probed a pilot version of the microarray with sera from individuals either acutely or chronically infected with S. mansoni from endemic areas in Brazil and sera from individuals resident outside the endemic area (USA) to determine if the array is functional and informative.

Idioma original	English (US)
Páginas (desde-hasta)	411-415
Número de páginas	5
Publicación	International Journal for Parasitology
Volumen	46
N.º	7
DOI	https://doi.org/10.1016/j.ijpara.2016.04.001
Estado	Published - jun 1 2016

ASJC Scopus subject areas

Parasitology
Infectious Diseases

Acceder al documento

10.1016/j.ijpara.2016.04.001

Otros archivos y enlaces

Citar esto

@article{57c66406145a4121a2e875ef02123d39,

title = "A next-generation proteome array for Schistosoma mansoni",

abstract = "A proteome microarray consisting of 992 Schistosoma mansoni proteins was produced and screened with sera to determine antibody signatures indicative of the clinical stages of schistosomiasis and the identification of subunit vaccine candidates. Herein, we describe the methods used to derive the gene list for this array (representing approximately 10% of the predicted S. mansoni proteome). We also probed a pilot version of the microarray with sera from individuals either acutely or chronically infected with S. mansoni from endemic areas in Brazil and sera from individuals resident outside the endemic area (USA) to determine if the array is functional and informative.",

keywords = "Antibody signature, Protein microarray, Proteome, Schistosoma mansoni",

author = "{de Assis}, {Rafael Ramiro} and Fernanda Ludolf and Rie Nakajima and Al Jasinskas and Oliveira, {Guilherme C.} and Felgner, {Philip L.} and Gaze, {Soraya T.} and Alex Loukas and LoVerde, {Philip T.} and Bethony, {Jeffrey M.} and Rodrigo Correa-Oliveira and Calzavara-Silva, {Carlos E.}",

note = "Funding Information: Funds for this project were from National Institutes of Health , USA, grant numbers U01 R01-AI095916-04 , U19-AI089686-05 and P50AI098507 , and from National Council for Scientific and Technological Development , Brazil, (CNPq) 478131/2012-7 , Rafael Ramiro de Assis was funded by CNPq ( 168260/2014-0 ). Publisher Copyright: {\textcopyright} 2016.",

year = "2016",

month = jun,

day = "1",

doi = "10.1016/j.ijpara.2016.04.001",

language = "English (US)",

volume = "46",

pages = "411--415",

journal = "International Journal for Parasitology",

issn = "0020-7519",

publisher = "Elsevier Limited",

number = "7",

}

TY - JOUR

T1 - A next-generation proteome array for Schistosoma mansoni

AU - de Assis, Rafael Ramiro

AU - Ludolf, Fernanda

AU - Nakajima, Rie

AU - Jasinskas, Al

AU - Oliveira, Guilherme C.

AU - Felgner, Philip L.

AU - Gaze, Soraya T.

AU - Loukas, Alex

AU - LoVerde, Philip T.

AU - Bethony, Jeffrey M.

AU - Correa-Oliveira, Rodrigo

AU - Calzavara-Silva, Carlos E.

N1 - Funding Information: Funds for this project were from National Institutes of Health , USA, grant numbers U01 R01-AI095916-04 , U19-AI089686-05 and P50AI098507 , and from National Council for Scientific and Technological Development , Brazil, (CNPq) 478131/2012-7 , Rafael Ramiro de Assis was funded by CNPq ( 168260/2014-0 ). Publisher Copyright: © 2016.

PY - 2016/6/1

Y1 - 2016/6/1

N2 - A proteome microarray consisting of 992 Schistosoma mansoni proteins was produced and screened with sera to determine antibody signatures indicative of the clinical stages of schistosomiasis and the identification of subunit vaccine candidates. Herein, we describe the methods used to derive the gene list for this array (representing approximately 10% of the predicted S. mansoni proteome). We also probed a pilot version of the microarray with sera from individuals either acutely or chronically infected with S. mansoni from endemic areas in Brazil and sera from individuals resident outside the endemic area (USA) to determine if the array is functional and informative.

AB - A proteome microarray consisting of 992 Schistosoma mansoni proteins was produced and screened with sera to determine antibody signatures indicative of the clinical stages of schistosomiasis and the identification of subunit vaccine candidates. Herein, we describe the methods used to derive the gene list for this array (representing approximately 10% of the predicted S. mansoni proteome). We also probed a pilot version of the microarray with sera from individuals either acutely or chronically infected with S. mansoni from endemic areas in Brazil and sera from individuals resident outside the endemic area (USA) to determine if the array is functional and informative.

KW - Antibody signature

KW - Protein microarray

KW - Proteome

KW - Schistosoma mansoni

UR - http://www.scopus.com/inward/record.url?scp=84969961367&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84969961367&partnerID=8YFLogxK

U2 - 10.1016/j.ijpara.2016.04.001

DO - 10.1016/j.ijpara.2016.04.001

M3 - Article

C2 - 27131510

AN - SCOPUS:84969961367

SN - 0020-7519

VL - 46

SP - 411

EP - 415

JO - International Journal for Parasitology

JF - International Journal for Parasitology

IS - 7

ER -

A next-generation proteome array for Schistosoma mansoni

Resumen

ASJC Scopus subject areas

Acceder al documento

Otros archivos y enlaces

Huella

Citar esto