A newly synthesized sinapic acid derivative inhibits endothelial activation in vitro and in vivos

Xiaoyun Zeng, Jinhong Zheng, Chenglai Fu, Hang Su, Xiaoli Sun, Xuesi Zhang, Yingjian Hou, Yi Zhu

Producción científica: Articlerevisión exhaustiva

15 Citas (Scopus)

Resumen

Inhibition of oxidative stress and inflammation in vascular endothelial cells (ECs) may represent a new therapeutic strategy against endothelial activation. Sinapic acid (SA), a phenylpropanoid compound, is found in natural herbs and high-bran cereals and has moderate antioxidant activity. We aimed to develop new SA agents with the properties of antioxidation and blocking EC activation for possible therapy of cardiovascular disease. We designed and synthesized 10 SA derivatives according to their chemical structures. Preliminary screening of the compounds involved scavenging hydroxyl radicals and 2,2-diphenyl-1- picrylhydrazyl (DPPH×), croton oil-induced ear edema in mice, and analysis of the mRNA expression of adhesion molecules in ECs. 1- Acetyl-sinapic acyl-4-(39-chlorine-)benzylpiperazine (SA9) had the strongest antioxidant and anti-inflammatory activities both in vitro and in vivo. Thus, the effect of SA9 was further studied. SA9 inhibited tumor necrosis factor a-induced upregulation of adhesion molecules in ECs at both mRNA and protein levels, as well as the consequent monocyte adhesion to ECs. In vivo, result of face-toface immunostaining showed that SA9 reduced lipopolysaccharideinduced expression of intercellular adhesion molecule-1 in mouse aortic intima. To study the molecular mechanism, results from luciferase assay, nuclear translocation of NF-kB, and Western blot indicated that the mechanism of the anti-inflammatory effects of SA9 might be suppression of intracellular generation of ROS and inhibition of NF-kB activation in ECs. SA9 is a prototype of a novel class of antioxidant with anti-inflammatory effects in ECs. It may represent a new therapeutic approach for preventing endothelial activation in cardiovascular disorders.

Idioma originalEnglish (US)
Páginas (desde-hasta)1099-1108
Número de páginas10
PublicaciónMolecular pharmacology
Volumen83
N.º5
DOI
EstadoPublished - may 2013
Publicado de forma externa

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

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