A high dose of ionizing radiation induces tissue-specific activation of nuclear factor-κB in vivo

Daohong Zhou, Stephen A. Brown, Tao Yu, Gang Chen, Shirish Barve, Bann C. Kang, John S. Thompson

Producción científica: Articlerevisión exhaustiva

75 Citas (Scopus)

Resumen

Activation of the transcription factor nuclear factor-κB (NF-κB) is one of the important responses of cells to an external stress such as ionizing radiation. We studied radiation-induced NF-κB activation in vivo in male BALB/c mice. After the mice were exposed to 8.5 Gy total-body γ irradiation, the spleen, mesenteric lymph nodes, thymus, liver, lung, colon, brain and bone marrow were harvested 1, 2.5, 5, 10 and 20 h postirradiation. NF-κB DNA-binding activity was analyzed in the nuclear protein extracts by a gel shift assay. When compared to the levels in untreated control mice, radiation induced activation of NF-κB in spleen, mesenteric lymph nodes and bone marrow but not in the other tissues examined. In contrast, an i.p. injection of a lethal dose (3 mg/kg) of lipopolysaccharide also increased activity of NF-κB in the liver and lung. The gel supershift assay with Nfkb1, Rela and/or Rel antibodies revealed that the specific molecular forms of NF-κB activated by radiation in the spleen were Nfkb1 homodimers and Nfkb1/Rela heterodimers. In mesenteric lymph nodes, the heterodimerized Rel/Rela NF-κB was also activated. In bone marrow, an NF-κB-like binding factor was induced that may be Nfkb1/Rela- and Rel/Rela-like heterodimers, but it exhibited a higher mobility than Nfkb1 homodimers. These results indicate that in vivo, ionizing radiation induces NF-κB activation that varies in both tissue distribution and molecular composition.

Idioma originalEnglish (US)
Páginas (desde-hasta)703-709
Número de páginas7
PublicaciónRadiation Research
Volumen151
N.º6
DOI
EstadoPublished - jun 1999
Publicado de forma externa

ASJC Scopus subject areas

  • Radiation
  • Biophysics
  • Radiology Nuclear Medicine and imaging

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