TY - JOUR
T1 - A Combination of a GnRH Antagonist and Agonist for Fertility Preservation in an Adolescent Female Murine Model
AU - Knudtson, Jennifer Flora
AU - Tellez Santos, Marlen
AU - Failor, Courtney M.
AU - Binkley, Peter A.
AU - Venesky, Jacob P.
AU - Tekmal, Rajeshwar R.
AU - Robinson, Randal D.
AU - Schenken, Robert S.
N1 - Publisher Copyright:
© The Author(s) 2016.
PY - 2017/9/1
Y1 - 2017/9/1
N2 - Recent studies have suggested that GnRH agonists (GnRHags) protect ovarian function following chemotherapy. Here, we study the effect of a combination of GnRH antagonist (GnRHan) and GnRHag for gonadal protection from gonadotoxic chemotherapy in adolescent female rats. Cycling Sprague Dawley rats were treated at adolescent age. Thirty female rats were randomized to 5 treatment groups (n = 6/group): (1) placebo, (2) cyclophosphamide (CPA) alone, (3) GnRHan followed by GnRHag with placebo, (4) GnRHan followed by GnRHag with CPA, and (5) GnRHag with CPA. The main outcome measure was live birth rate (LBR), and secondary measures included rat weight, ovarian volume, and follicles. Group 2 had decreased LBR compared to all other groups. Group 4 and 5 had LBR similar to placebo. There was no difference in the ovarian volume. The CPA-alone group had decreased number of antral follicles compared to control. These studies demonstrate that the combination of GnRHan and GnRHag and GnRHag alone preserved fertility in female adolescent rats following gonadotoxic chemotherapy treatment. The addition of a GnRHan to a GnRHag does not confer a greater protective effect.
AB - Recent studies have suggested that GnRH agonists (GnRHags) protect ovarian function following chemotherapy. Here, we study the effect of a combination of GnRH antagonist (GnRHan) and GnRHag for gonadal protection from gonadotoxic chemotherapy in adolescent female rats. Cycling Sprague Dawley rats were treated at adolescent age. Thirty female rats were randomized to 5 treatment groups (n = 6/group): (1) placebo, (2) cyclophosphamide (CPA) alone, (3) GnRHan followed by GnRHag with placebo, (4) GnRHan followed by GnRHag with CPA, and (5) GnRHag with CPA. The main outcome measure was live birth rate (LBR), and secondary measures included rat weight, ovarian volume, and follicles. Group 2 had decreased LBR compared to all other groups. Group 4 and 5 had LBR similar to placebo. There was no difference in the ovarian volume. The CPA-alone group had decreased number of antral follicles compared to control. These studies demonstrate that the combination of GnRHan and GnRHag and GnRHag alone preserved fertility in female adolescent rats following gonadotoxic chemotherapy treatment. The addition of a GnRHan to a GnRHag does not confer a greater protective effect.
KW - GnRH suppression
KW - adolescents (AYA)
KW - fertility preservation
UR - http://www.scopus.com/inward/record.url?scp=85027692236&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85027692236&partnerID=8YFLogxK
U2 - 10.1177/1933719116682876
DO - 10.1177/1933719116682876
M3 - Article
C2 - 28290768
AN - SCOPUS:85027692236
SN - 1933-7191
VL - 24
SP - 1280
EP - 1283
JO - Reproductive Sciences
JF - Reproductive Sciences
IS - 9
ER -