TY - JOUR
T1 - β-Carotene-mediated inhibition of a DNA adduct induced by 7,12-dimethylbenz(a)anthracene and 7-hydroxymethyl-12-methylbenz(a)anthracene in mouse mammary gland in vitro
AU - Das, Sanjoy K.
AU - Jia, Ting Z.
AU - Bandyopadhyay, Abhik M.
AU - Banerjee, Mihir R.
N1 - Funding Information:
Acknowledgements-We thank M. Mathiesen,R . Schmaltza nd J. Treves for their technicala ssistanceT. his work was supported by USPHS, NationalC ancerI nstituteg rantC A25304t o M.R. Banerjee. We alsot hankD onnaM . McCarthyf or here ditoriaal ssistance.
PY - 1992/5
Y1 - 1992/5
N2 - The influence of β-carotene on the formation of DNA-adducts induced by 7,12-dimethylbenz(a)anthracene (DMBA) and 7-hydroxymethyl-12-methylbenz(a)anthracene (7-OHM-12-MBA) during transformation of mouse mammary cells in organ culture was analysed. Treatment with β-carotene (10-8-10-5 mol/l) caused inhibition (48.8-94.4%) of an adduct (VI), which was detectable in DNA samples from DMBA-treated mammary glands. Out of six adducts, derived from further analysis of DNA samples from 7-OHM-12-MBA-treated glands, adduct f eluted in the same fraction as adduct (VI), indicating these adducts were analogous. Likewise, adduct f was also inhibited by β-carotene. Boronate chromatographic analysis revealed this particular adduct was a syn-dihydrodiol epoxide product. Adduct inhibition was detectable both at the start and after DMBA treatment. α-Tocopherol and canthaxanthin were ineffective in inhibiting adducts. It is reasonable to conclude that β-carotene-mediated modification of adducts is associated with the inhibition of a syn-adduct, which is derived from further metabolism of a 7-OHM-12-MBA intermediate.
AB - The influence of β-carotene on the formation of DNA-adducts induced by 7,12-dimethylbenz(a)anthracene (DMBA) and 7-hydroxymethyl-12-methylbenz(a)anthracene (7-OHM-12-MBA) during transformation of mouse mammary cells in organ culture was analysed. Treatment with β-carotene (10-8-10-5 mol/l) caused inhibition (48.8-94.4%) of an adduct (VI), which was detectable in DNA samples from DMBA-treated mammary glands. Out of six adducts, derived from further analysis of DNA samples from 7-OHM-12-MBA-treated glands, adduct f eluted in the same fraction as adduct (VI), indicating these adducts were analogous. Likewise, adduct f was also inhibited by β-carotene. Boronate chromatographic analysis revealed this particular adduct was a syn-dihydrodiol epoxide product. Adduct inhibition was detectable both at the start and after DMBA treatment. α-Tocopherol and canthaxanthin were ineffective in inhibiting adducts. It is reasonable to conclude that β-carotene-mediated modification of adducts is associated with the inhibition of a syn-adduct, which is derived from further metabolism of a 7-OHM-12-MBA intermediate.
UR - http://www.scopus.com/inward/record.url?scp=0026741247&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026741247&partnerID=8YFLogxK
U2 - 10.1016/0959-8049(92)90470-M
DO - 10.1016/0959-8049(92)90470-M
M3 - Article
C2 - 1627382
AN - SCOPUS:0026741247
SN - 0959-8049
VL - 28
SP - 1124
EP - 1129
JO - European Journal of Cancer
JF - European Journal of Cancer
IS - 6-7
ER -