Description
Accession Number: GSE60270
Platform:
GPL10999: Illumina Genome Analyzer IIx (Homo sapiens)
GPL11154: Illumina HiSeq 2000 (Homo sapiens)
Organism: Homo sapiens
Published on 2014-10-09
Summary:
Enhancers provide critical information directing cell-type specific transcriptional programs, regulated by binding of signal-dependent transcription factors and their associated cofactors. Here we report that the most strongly activated estrogen (E2)-responsive enhancers are characterized by trans-recruitment and in situ assembly of a large 1-2MDa complex of diverse DNA-binding transcription factors by ERα at ERE-containing enhancers. We refer to enhancers recruiting these factors as mega transcription factor-bound in trans (MegaTrans) enhancers. The MegaTrans complex is a signature of the most potent functional enhancers and is required for activation of enhancer RNA transcription and recruitment of coactivators, including p300 and Med1. The MegaTrans complex functions, in part, by recruiting specific enzymatic machinery, exemplified by DNA-dependent protein kinase. Thus, MegaTrans-containing enhancers represent a cohort of functional enhancers that mediate a broad and important transcriptional program and provide a molecular explanation for transcription factor clustering and hotspots noted in the genome.
Overall Design:
Chromatin Immunoprecipitation (ChIP) assay followed by high throughput sequencing (ChIP-seq)
Contact:
Name: Daria Merkurjev
Organization: UCSD
Laboratory: Michael G. Rosenfeld Laboratory
Deparment: Medicine
Address: 9500 Gilman Drive, Mail Code 0648 La Jolla CA 92093 USA
Email: [email protected]
Phone: 858-534-5858
Organization: GEO
Address: USA
Platform:
GPL10999: Illumina Genome Analyzer IIx (Homo sapiens)
GPL11154: Illumina HiSeq 2000 (Homo sapiens)
Organism: Homo sapiens
Published on 2014-10-09
Summary:
Enhancers provide critical information directing cell-type specific transcriptional programs, regulated by binding of signal-dependent transcription factors and their associated cofactors. Here we report that the most strongly activated estrogen (E2)-responsive enhancers are characterized by trans-recruitment and in situ assembly of a large 1-2MDa complex of diverse DNA-binding transcription factors by ERα at ERE-containing enhancers. We refer to enhancers recruiting these factors as mega transcription factor-bound in trans (MegaTrans) enhancers. The MegaTrans complex is a signature of the most potent functional enhancers and is required for activation of enhancer RNA transcription and recruitment of coactivators, including p300 and Med1. The MegaTrans complex functions, in part, by recruiting specific enzymatic machinery, exemplified by DNA-dependent protein kinase. Thus, MegaTrans-containing enhancers represent a cohort of functional enhancers that mediate a broad and important transcriptional program and provide a molecular explanation for transcription factor clustering and hotspots noted in the genome.
Overall Design:
Chromatin Immunoprecipitation (ChIP) assay followed by high throughput sequencing (ChIP-seq)
Contact:
Name: Daria Merkurjev
Organization: UCSD
Laboratory: Michael G. Rosenfeld Laboratory
Deparment: Medicine
Address: 9500 Gilman Drive, Mail Code 0648 La Jolla CA 92093 USA
Email: [email protected]
Phone: 858-534-5858
Organization: GEO
Address: USA
| Datos disponibles | ago 8 2014 |
|---|---|
| Editor | Gene Expression Omnibus |