Zebrafish Tumor Graft Transplantation to Grow Tumors in Vivo That Engraft Poorly as Single Cell Suspensions

Amanda Lipsitt, Nicole R. Hensch, Rodrigo Moreno-Campos, Kunal Baxi, Jiangfei Chen, Anil K. Challa, Eleanor Y. Chen, Myron S. Ignatius

Research output: Contribution to journalArticlepeer-review

Abstract

Angiosarcoma is a clinically aggressive tumor with a high rate of mortality. It can arise in vascular or lymphatic tissues, involve any part of the body, and aggressively spread locally or metastasize. Angiosarcomas spontaneously develop in the tp53 deleted (tp53del/del) zebrafish mutant. However, established protocols for tumor dissection and transplantation of single cell suspensions of angiosarcoma tumors result in inferior implantation rates. To resolve these complications, we developed a new tumor grafting technique for engraftment of angiosarcoma and similar tumors in zebrafish, which maintains the tumor microenvironment and has superior rates of engraftment.

Original languageEnglish (US)
Pages (from-to)293-296
Number of pages4
JournalZebrafish
Volume18
Issue number4
DOIs
StatePublished - Aug 2021

Keywords

  • angiosarcoma
  • tp53
  • tumor graft
  • zebrafish
  • zebrafish cancer model

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Developmental Biology

Fingerprint

Dive into the research topics of 'Zebrafish Tumor Graft Transplantation to Grow Tumors in Vivo That Engraft Poorly as Single Cell Suspensions'. Together they form a unique fingerprint.

Cite this