Zebrafish Tumor Graft Transplantation to Grow Tumors in Vivo That Engraft Poorly as Single Cell Suspensions

Amanda Lipsitt; Nicole R. Hensch; Rodrigo Moreno-Campos; Kunal Baxi; Jiangfei Chen; Anil K. Challa; Eleanor Y. Chen; Myron S. Ignatius

doi:10.1089/zeb.2021.0006

Zebrafish Tumor Graft Transplantation to Grow Tumors in Vivo That Engraft Poorly as Single Cell Suspensions

Amanda Lipsitt, Nicole R. Hensch, Rodrigo Moreno-Campos, Kunal Baxi, Jiangfei Chen, Anil K. Challa, Eleanor Y. Chen, Myron S. Ignatius

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Angiosarcoma is a clinically aggressive tumor with a high rate of mortality. It can arise in vascular or lymphatic tissues, involve any part of the body, and aggressively spread locally or metastasize. Angiosarcomas spontaneously develop in the tp53 deleted (tp53del/del) zebrafish mutant. However, established protocols for tumor dissection and transplantation of single cell suspensions of angiosarcoma tumors result in inferior implantation rates. To resolve these complications, we developed a new tumor grafting technique for engraftment of angiosarcoma and similar tumors in zebrafish, which maintains the tumor microenvironment and has superior rates of engraftment.

Original language	English (US)
Pages (from-to)	293-296
Number of pages	4
Journal	Zebrafish
Volume	18
Issue number	4
DOIs	https://doi.org/10.1089/zeb.2021.0006
State	Published - Aug 2021

Keywords

angiosarcoma
tp53
tumor graft
zebrafish
zebrafish cancer model

ASJC Scopus subject areas

Animal Science and Zoology
Developmental Biology

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10.1089/zeb.2021.0006

Cite this

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title = "Zebrafish Tumor Graft Transplantation to Grow Tumors in Vivo That Engraft Poorly as Single Cell Suspensions",

abstract = "Angiosarcoma is a clinically aggressive tumor with a high rate of mortality. It can arise in vascular or lymphatic tissues, involve any part of the body, and aggressively spread locally or metastasize. Angiosarcomas spontaneously develop in the tp53 deleted (tp53del/del) zebrafish mutant. However, established protocols for tumor dissection and transplantation of single cell suspensions of angiosarcoma tumors result in inferior implantation rates. To resolve these complications, we developed a new tumor grafting technique for engraftment of angiosarcoma and similar tumors in zebrafish, which maintains the tumor microenvironment and has superior rates of engraftment.",

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year = "2021",

month = aug,

doi = "10.1089/zeb.2021.0006",

language = "English (US)",

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journal = "Zebrafish",

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T1 - Zebrafish Tumor Graft Transplantation to Grow Tumors in Vivo That Engraft Poorly as Single Cell Suspensions

AU - Lipsitt, Amanda

AU - Hensch, Nicole R.

AU - Moreno-Campos, Rodrigo

AU - Baxi, Kunal

AU - Chen, Jiangfei

AU - Challa, Anil K.

AU - Chen, Eleanor Y.

AU - Ignatius, Myron S.

PY - 2021/8

Y1 - 2021/8

N2 - Angiosarcoma is a clinically aggressive tumor with a high rate of mortality. It can arise in vascular or lymphatic tissues, involve any part of the body, and aggressively spread locally or metastasize. Angiosarcomas spontaneously develop in the tp53 deleted (tp53del/del) zebrafish mutant. However, established protocols for tumor dissection and transplantation of single cell suspensions of angiosarcoma tumors result in inferior implantation rates. To resolve these complications, we developed a new tumor grafting technique for engraftment of angiosarcoma and similar tumors in zebrafish, which maintains the tumor microenvironment and has superior rates of engraftment.

AB - Angiosarcoma is a clinically aggressive tumor with a high rate of mortality. It can arise in vascular or lymphatic tissues, involve any part of the body, and aggressively spread locally or metastasize. Angiosarcomas spontaneously develop in the tp53 deleted (tp53del/del) zebrafish mutant. However, established protocols for tumor dissection and transplantation of single cell suspensions of angiosarcoma tumors result in inferior implantation rates. To resolve these complications, we developed a new tumor grafting technique for engraftment of angiosarcoma and similar tumors in zebrafish, which maintains the tumor microenvironment and has superior rates of engraftment.

KW - angiosarcoma

KW - tp53

KW - tumor graft

KW - zebrafish

KW - zebrafish cancer model

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Zebrafish Tumor Graft Transplantation to Grow Tumors in Vivo That Engraft Poorly as Single Cell Suspensions

Abstract

Keywords

ASJC Scopus subject areas

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