@article{2dfcb66c45d24f958147ad4d49045689,
title = "Yeast artificial chromosome (YAC) -based genome mapping of Schistosoma mansoni",
abstract = "Schistosoma mansoni has 7 pairs of autosomal chromosomes and one pair of sex chromosomes (ZZ for a male worm and ZW for a female), of a haploid genome size of 2.7 × 108 bp. We initiated the molecular genetic approach for the detailed characterization and understanding of the evolutionary biology of schistosomes. We have constructed a yeast artificial chromosome (YAC)-library with partially digested parasite genomic DNA, and the chromosome location of each insert was detected by fluorescent in situ hybridization (FISH). The library contains > 2283 clones with an average insert size of 358 kb, which represents a 2.6-fold coverage of the genome (> 7.2 × 108 bp). 100 randomly selected YAC clones were localized by FISH and found to be distributed widely among all chromosomes. The assembly of 14 YACs distributed almost the whole region of chromosome 3. Generated expressed sequenced tags (ESTs) derived from a unidirectional cDNA library were also used for further characterization of the YAC inserts. These results indicate that an extensive contig assembly of the entire chromosomes and a reasonably detailed gene map should be feasible in the near future.",
keywords = "Expressed sequence tag, Fluorescent in situ hybridisation, Genome mapping, Schistosoma mansoni, Yeast artificial chromosome",
author = "Manami Tanaka and Hirohisa Hirai and LoVerde, {Philip T.} and Shigeo Nagafuchi and Franco, {Gl{\'o}ria R.} and Simpson, {Andrew J.G.} and Pena, {S{\'e}rgio D.J.}",
note = "Funding Information: a Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Ibaraki 305, Japan b Primate Research Institute, Kyoto University, Inuyama, Aichi 484, Japan {\textquoteright} Department of Microbiology, School of Medicine and Biomedical Sciences, State Universiry of New York at Buffalo, Buffalo, NY 14214, USA {\textquoteright} National Children s Medical Research Center, 3-35-31 Taishido, Setagaya, Tokyo 1.54, Japan {\textquoteright} Departamento de Bioquimica, Vniuersidade Federal de Minas Gerais, Belo Horizonte ,31270-901, Brazil f Centro de Pesquisas {\textquoteleft}Renk Rachou{\textquoteright}, Funda@o Oswaid Cruz, Minas Gerais, Belo Hortionte, 30190-002 Brazil Funding Information: We thankM r. NobuhikoS hirato,M r. Kouji Sae-gusa,M s. Toshie Sawadaa nd Mr. Kozo Kobarashi for technicaal ssistanceD;r s. TakashiI mai, Schizhen Qin, and Masao Yamadaf or much valueda dvice; Dr. Momoki Hirai for the two-color FISH techniques;M r. Hiromi Tamahidef or image-analyzing the double-colorF ISH. The work was supportedb y grants from Ministry of Education,S cience, and Culture, Japan (Nos. 06044032, 06670253, 05670222)N; IH grant (AI 188671U, SA, Nissan ScienceF oundationJ,a pan;Y amadaS cienceF ounda-tion,J apan:I chiro KanaharaF oundationJ,a pan;U ni-versityo f TsukubaP roject.",
year = "1995",
month = jan,
doi = "10.1016/0166-6851(94)00182-M",
language = "English (US)",
volume = "69",
pages = "41--51",
journal = "Molecular and Biochemical Parasitology",
issn = "0166-6851",
publisher = "Elsevier",
number = "1",
}