Yeast artificial chromosome (YAC) -based genome mapping of Schistosoma mansoni

Manami Tanaka, Hirohisa Hirai, Philip T. LoVerde, Shigeo Nagafuchi, Glória R. Franco, Andrew J.G. Simpson, Sérgio D.J. Pena

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

Schistosoma mansoni has 7 pairs of autosomal chromosomes and one pair of sex chromosomes (ZZ for a male worm and ZW for a female), of a haploid genome size of 2.7 × 108 bp. We initiated the molecular genetic approach for the detailed characterization and understanding of the evolutionary biology of schistosomes. We have constructed a yeast artificial chromosome (YAC)-library with partially digested parasite genomic DNA, and the chromosome location of each insert was detected by fluorescent in situ hybridization (FISH). The library contains > 2283 clones with an average insert size of 358 kb, which represents a 2.6-fold coverage of the genome (> 7.2 × 108 bp). 100 randomly selected YAC clones were localized by FISH and found to be distributed widely among all chromosomes. The assembly of 14 YACs distributed almost the whole region of chromosome 3. Generated expressed sequenced tags (ESTs) derived from a unidirectional cDNA library were also used for further characterization of the YAC inserts. These results indicate that an extensive contig assembly of the entire chromosomes and a reasonably detailed gene map should be feasible in the near future.

Original languageEnglish (US)
Pages (from-to)41-51
Number of pages11
JournalMolecular and Biochemical Parasitology
Volume69
Issue number1
DOIs
StatePublished - Jan 1995

Keywords

  • Expressed sequence tag
  • Fluorescent in situ hybridisation
  • Genome mapping
  • Schistosoma mansoni
  • Yeast artificial chromosome

ASJC Scopus subject areas

  • Parasitology
  • Molecular Biology

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