Wild-type MIC distributions and epidemiological cutoff values for amphotericin B and aspergillus spp. for the CLSI broth microdilution method (M38-A2 document)

A. Espinel-Ingroff, M. Cuenca-Estrella, A. Fothergill, J. Fuller, M. Ghannoum, E. Johnson, T. Pelaez, M. A. Pfaller, J. Turnidge

Research output: Contribution to journalArticle

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Abstract

Although clinical breakpoints have not been established for mold testing, epidemiological cutoff values (ECVs) are available for Aspergillus spp. versus the triazoles and caspofungin. Wild-type (WT) MIC distributions (organisms in a species-drug combination with no acquired resistance mechanisms) were defined in order to establish ECVs for six Aspergillus spp. and amphotericin B. Two sets (CLSI/EUCAST broth microdilution) of available MICs were evaluated: those for A. fumigatus (3,988/833), A. flavus (793/194), A. nidulans (184/69), A. niger (673/140), A. terreus (545/266), and A. versicolor (135/22). Three sets of data were analyzed: (i) CLSI data gathered in eight independent laboratories in Canada, Europe, and the United States; (ii) EUCAST data from a single laboratory; and (iii) the combined CLSI and EUCAST data. ECVs, expressed in μg/ml, that captured 95%, 97.5%, and 99% of the modeled wild-type population (CLSI and combined data) were as follows: for A. fumigatus, 2, 2, and 4; for A. flavus, 2, 4, and 4; for A. nidulans, 4, 4, and 4; for A. niger, 2, 2, and 2; for A. terreus, 4, 4, and 8; and for A. versicolor, 2, 2, and 2. Similar to the case for the triazoles and caspofungin, amphotericin B ECVs may aid in the detection of strains with acquired mechanisms of resistance to this agent.

Original languageEnglish (US)
Pages (from-to)5150-5154
Number of pages5
JournalAntimicrobial Agents and Chemotherapy
Volume55
Issue number11
DOIs
StatePublished - Nov 2011

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caspofungin
Triazoles
Amphotericin B
Aspergillus
varespladib methyl
Drug Combinations
Canada
Fungi
Population

ASJC Scopus subject areas

  • Pharmacology (medical)
  • Pharmacology
  • Infectious Diseases

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Wild-type MIC distributions and epidemiological cutoff values for amphotericin B and aspergillus spp. for the CLSI broth microdilution method (M38-A2 document). / Espinel-Ingroff, A.; Cuenca-Estrella, M.; Fothergill, A.; Fuller, J.; Ghannoum, M.; Johnson, E.; Pelaez, T.; Pfaller, M. A.; Turnidge, J.

In: Antimicrobial Agents and Chemotherapy, Vol. 55, No. 11, 11.2011, p. 5150-5154.

Research output: Contribution to journalArticle

Espinel-Ingroff, A, Cuenca-Estrella, M, Fothergill, A, Fuller, J, Ghannoum, M, Johnson, E, Pelaez, T, Pfaller, MA & Turnidge, J 2011, 'Wild-type MIC distributions and epidemiological cutoff values for amphotericin B and aspergillus spp. for the CLSI broth microdilution method (M38-A2 document)', Antimicrobial Agents and Chemotherapy, vol. 55, no. 11, pp. 5150-5154. https://doi.org/10.1128/AAC.00686-11
Espinel-Ingroff, A. ; Cuenca-Estrella, M. ; Fothergill, A. ; Fuller, J. ; Ghannoum, M. ; Johnson, E. ; Pelaez, T. ; Pfaller, M. A. ; Turnidge, J. / Wild-type MIC distributions and epidemiological cutoff values for amphotericin B and aspergillus spp. for the CLSI broth microdilution method (M38-A2 document). In: Antimicrobial Agents and Chemotherapy. 2011 ; Vol. 55, No. 11. pp. 5150-5154.
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AU - Ghannoum, M.

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