Whole body proton irradiation causes acute damage to bone marrow hematopoietic progenitor and stem cells in mice

Jianhui Chang, Yingying Wang, Rupak Pathak, Vijayalakshmi Sridharan, Tamako Jones, Xiao Wen Mao, Gregory Nelson, Marjan Boerma, Martin Hauer-Jensen, Daohong Zhou, Lijian Shao

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Purpose: Exposure to proton irradiation during missions in deep space can lead to bone marrow injury. The acute effects of proton irradiation on hematopoietic stem and progenitor cells remain undefined and thus were investigated. Materials and methods: We exposed male C57BL/6 mice to 0.5 and 1.0 Gy proton total body irradiation (proton-TBI, 150 MeV) and examined changes in peripheral blood cells and bone marrow (BM) progenitors and LSK cells 2 weeks after exposure. Results: 1.0 Gy proton-TBI significantly reduced the numbers of peripheral blood cells compared to 0.5 Gy proton-TBI and unirradiated animals, while the numbers of peripheral blood cell counts were comparable between 0.5 Gy proton-TBI and unirradiated mice. The frequencies and numbers of LSK cells and CMPs in BM of 0.5 and 1.0 Gy irradiated mice were decreased in comparison to those of normal controls. LSK cells and CMPs and their progeny exhibited a radiation-induced impairment in clonogenic function. Exposure to 1.0 Gy increased cellular apoptosis but not the production of reactive oxygen species (ROS) in CMPs two weeks after irradiation. LSK cells from irradiated mice exhibited an increase in ROS production and apoptosis. Conclusion: Exposure to proton-TBI can induce acute damage to BM progenitors and LSK cells.

Original languageEnglish (US)
Pages (from-to)1312-1320
Number of pages9
JournalInternational Journal of Radiation Biology
Volume93
Issue number12
DOIs
StatePublished - Dec 2 2017
Externally publishedYes

Keywords

  • apoptosis
  • hematopoietic stem cells
  • Proton irradiation
  • reactive oxygen species

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology
  • Radiology Nuclear Medicine and imaging

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