What causes canine sino-nasal aspergillosis? A molecular approach to species identification

Jessica J. Talbot; Lynelle R. Johnson; Patricia Martin; Julia A. Beatty; Deanna A. Sutton; Frédéric Billen; Catriona L. Halliday; Justine S. Gibson; Sarah Kidd; Jörg M. Steiner; Beata Ujvari; Vanessa R. Barrs

doi:10.1016/j.tvjl.2014.01.009

What causes canine sino-nasal aspergillosis? A molecular approach to species identification

Jessica J. Talbot, Lynelle R. Johnson, Patricia Martin, Julia A. Beatty, Deanna A. Sutton, Frédéric Billen, Catriona L. Halliday, Justine S. Gibson, Sarah Kidd, Jörg M. Steiner, Beata Ujvari, Vanessa R. Barrs

Department of Pathology

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

On the basis of phenotypic identification methods, Aspergillus fumigatus is reported as the most commonly identified aetiological agent of canine sino-nasal aspergillosis (SNA). However, definitive identification of Aspergillus spp. using phenotypic features alone is unreliable. The aim of this study was to determine the molecular identities of fungal species causing SNA in dogs. Genomic DNA was extracted from 91 fungal isolates from 90 dogs diagnosed with SNA in Australia, the USA and Belgium, and the ITS1-5.8S-ITS2 ribosomal DNA and partial β-tubulin regions were sequenced. Eighty-eight of 91 (96.7%) isolates were identified as A. fumigatus and 3/91 (3.3%) belonged to Aspergillus section Nigri spp. (Aspergillus tubingensis: 2/91; Aspergillus uvarum: 1/91). These findings confirm that A. fumigatus is the most common aetiological agent of canine SNA. This is the first report to document a pathogenic role for A. tubingensis and A. uvarum in dogs.

Original language	English (US)
Pages (from-to)	17-21
Number of pages	5
Journal	Veterinary Journal
Volume	200
Issue number	1
DOIs	https://doi.org/10.1016/j.tvjl.2014.01.009
State	Published - Apr 2014

Keywords

Aspergillus fumigatus
Aspergillus tubingensis
Aspergillus uvarum
Canine
Sino-nasal aspergillosis

ASJC Scopus subject areas

Animal Science and Zoology
veterinary(all)

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10.1016/j.tvjl.2014.01.009

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title = "What causes canine sino-nasal aspergillosis? A molecular approach to species identification",

abstract = "On the basis of phenotypic identification methods, Aspergillus fumigatus is reported as the most commonly identified aetiological agent of canine sino-nasal aspergillosis (SNA). However, definitive identification of Aspergillus spp. using phenotypic features alone is unreliable. The aim of this study was to determine the molecular identities of fungal species causing SNA in dogs. Genomic DNA was extracted from 91 fungal isolates from 90 dogs diagnosed with SNA in Australia, the USA and Belgium, and the ITS1-5.8S-ITS2 ribosomal DNA and partial β-tubulin regions were sequenced. Eighty-eight of 91 (96.7%) isolates were identified as A. fumigatus and 3/91 (3.3%) belonged to Aspergillus section Nigri spp. (Aspergillus tubingensis: 2/91; Aspergillus uvarum: 1/91). These findings confirm that A. fumigatus is the most common aetiological agent of canine SNA. This is the first report to document a pathogenic role for A. tubingensis and A. uvarum in dogs.",

keywords = "Aspergillus fumigatus, Aspergillus tubingensis, Aspergillus uvarum, Canine, Sino-nasal aspergillosis",

author = "Talbot, {Jessica J.} and Johnson, {Lynelle R.} and Patricia Martin and Beatty, {Julia A.} and Sutton, {Deanna A.} and Fr{\'e}d{\'e}ric Billen and Halliday, {Catriona L.} and Gibson, {Justine S.} and Sarah Kidd and Steiner, {J{\"o}rg M.} and Beata Ujvari and Barrs, {Vanessa R.}",

note = "Funding Information: Project funding was received from the Veterinary Science Foundation Canine and Feline Fund and the Thea Jean Schnakenberg bequest from the Faculty of Veterinary Science, University of Sydney. We thank Professor Kathy Belov for sharing laboratory facilities for this project. The authors wish to acknowledge veterinary clinicians at participating institutions and the Small Animal Specialist Hospital, Sydney and North Shore Veterinary Hospital, Sydney, Australia, for contributing samples. ",

year = "2014",

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doi = "10.1016/j.tvjl.2014.01.009",

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AU - Johnson, Lynelle R.

AU - Martin, Patricia

AU - Beatty, Julia A.

AU - Sutton, Deanna A.

AU - Billen, Frédéric

AU - Halliday, Catriona L.

AU - Gibson, Justine S.

AU - Kidd, Sarah

AU - Steiner, Jörg M.

AU - Ujvari, Beata

AU - Barrs, Vanessa R.

N1 - Funding Information: Project funding was received from the Veterinary Science Foundation Canine and Feline Fund and the Thea Jean Schnakenberg bequest from the Faculty of Veterinary Science, University of Sydney. We thank Professor Kathy Belov for sharing laboratory facilities for this project. The authors wish to acknowledge veterinary clinicians at participating institutions and the Small Animal Specialist Hospital, Sydney and North Shore Veterinary Hospital, Sydney, Australia, for contributing samples.

PY - 2014/4

Y1 - 2014/4

N2 - On the basis of phenotypic identification methods, Aspergillus fumigatus is reported as the most commonly identified aetiological agent of canine sino-nasal aspergillosis (SNA). However, definitive identification of Aspergillus spp. using phenotypic features alone is unreliable. The aim of this study was to determine the molecular identities of fungal species causing SNA in dogs. Genomic DNA was extracted from 91 fungal isolates from 90 dogs diagnosed with SNA in Australia, the USA and Belgium, and the ITS1-5.8S-ITS2 ribosomal DNA and partial β-tubulin regions were sequenced. Eighty-eight of 91 (96.7%) isolates were identified as A. fumigatus and 3/91 (3.3%) belonged to Aspergillus section Nigri spp. (Aspergillus tubingensis: 2/91; Aspergillus uvarum: 1/91). These findings confirm that A. fumigatus is the most common aetiological agent of canine SNA. This is the first report to document a pathogenic role for A. tubingensis and A. uvarum in dogs.

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What causes canine sino-nasal aspergillosis? A molecular approach to species identification

Abstract

Keywords

ASJC Scopus subject areas

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