Vitamin D heritability and effect of pregnancy status in Vervet monkeys (Chlorocebus aethiops sabaeus) under conditions of modest and high dietary supplementation

Geetha Chittoor, Nicholas M. Pajewski, V. Saroja Voruganti, Anthony G. Comuzzie, Thomas B. Clarkson, Matthew Nudy, Peter F. Schnatz, Jay R. Kaplan, Matthew J. Jorgensen

    Research output: Contribution to journalArticle

    3 Scopus citations

    Abstract

    Objectives The two objectives of the current study were to: 1) investigate the genetic contributions to variations in serum vitamin D concentrations under two dietary conditions (a standard monkey biscuit diet vs. a diet designed to model typical American consumption); and 2) explore the interaction of vitamin D with pregnancy status using a cohort of pedigreed female vervet/African green monkeys. Methods This study includes 185 female (≥3.5 years) vervet/African green monkeys (Chlorocebus aethiops sabaeus) from a multi-generational, pedigreed breeding colony. The 25(OH)D3 concentrations were first measured seven to eight weeks after consuming a "typical American" diet (TAD), deriving 37, 18, and 45% of calories from fat, protein sources, and carbohydrates, and supplemented with vitamin D to a human equivalent of 1,000 IU/day. Vitamin D concentrations were assessed again when animals were switched to a low-fat, standard biscuit diet (LabDiet 5038) for 8 months, which provided a human equivalent of approximately 4,000 IU/day of vitamin D. All statistical analyses were implemented in SOLAR. Results Pregnancy was associated with reduced 25(OH)D3 concentrations. Heritability analyses indicated a significant genetic contribution to 25(OH)D3 concentrations in the same monkeys consuming the biscuit diet (h2=0.66, P=0.0004) and TAD (h2=0.67, P=0.0078) diets, with higher 25(OH)D3 concentrations in animals consuming the biscuit diet. Additionally, there was a significant genotype-by-pregnancy status interaction on 25(OH)D3 concentrations (P<0.05) only among animals consuming the TAD diet. Discussion These results support the existence of a genetic contribution to differences in serum 25(OH)D3 concentrations by pregnancy status and emphasize the role of diet (including vitamin D supplementation) in modifying genetic signals as well as vitamin D concentrations.

    Original languageEnglish (US)
    Pages (from-to)639-645
    Number of pages7
    JournalAmerican Journal of Physical Anthropology
    Volume159
    Issue number4
    DOIs
    StatePublished - Apr 1 2016

    Keywords

    • 25(OH)D3
    • Vervet/African green monkeys
    • genotype-by pregnancy
    • genotype-by-diet

    ASJC Scopus subject areas

    • Anatomy
    • Anthropology

    Fingerprint Dive into the research topics of 'Vitamin D heritability and effect of pregnancy status in Vervet monkeys (Chlorocebus aethiops sabaeus) under conditions of modest and high dietary supplementation'. Together they form a unique fingerprint.

  • Cite this

    Chittoor, G., Pajewski, N. M., Voruganti, V. S., Comuzzie, A. G., Clarkson, T. B., Nudy, M., Schnatz, P. F., Kaplan, J. R., & Jorgensen, M. J. (2016). Vitamin D heritability and effect of pregnancy status in Vervet monkeys (Chlorocebus aethiops sabaeus) under conditions of modest and high dietary supplementation. American Journal of Physical Anthropology, 159(4), 639-645. https://doi.org/10.1002/ajpa.22923