A sensitive, new enzymatic method for the detection of isozymes which liberate inorganic phosphate (or pyrophosphate) is described. The new method for the detection of phosphate differs from the established method for nucleoside phosphorylase by the substitution of one reagent. The new enzymatic method, compared to existing methods for the detection of phosphate on electrophoretic gels, is advantageous due to its sensitivity and generation of a nondiffusible formazan chromogenic product.
|Original language||English (US)|
|Number of pages||6|
|State||Published - Oct 1981|
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics
- Molecular Biology