Visualization of isozymes which generate inorganic phosphate

Robert J. Klebe; Sindi Schloss; Lisa Mock; Carol R. Link

doi:10.1007/BF00504257

Visualization of isozymes which generate inorganic phosphate

Robert J. Klebe, Sindi Schloss, Lisa Mock, Carol R. Link

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

A sensitive, new enzymatic method for the detection of isozymes which liberate inorganic phosphate (or pyrophosphate) is described. The new method for the detection of phosphate differs from the established method for nucleoside phosphorylase by the substitution of one reagent. The new enzymatic method, compared to existing methods for the detection of phosphate on electrophoretic gels, is advantageous due to its sensitivity and generation of a nondiffusible formazan chromogenic product.

Original language	English (US)
Pages (from-to)	921-926
Number of pages	6
Journal	Biochemical Genetics
Volume	19
Issue number	9-10
DOIs	https://doi.org/10.1007/BF00504257
State	Published - Oct 1981
Externally published	Yes

Keywords

electrophoresis
isozymes
phosphate

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Biochemistry
Molecular Biology
Genetics

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10.1007/BF00504257

Cite this

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title = "Visualization of isozymes which generate inorganic phosphate",

abstract = "A sensitive, new enzymatic method for the detection of isozymes which liberate inorganic phosphate (or pyrophosphate) is described. The new method for the detection of phosphate differs from the established method for nucleoside phosphorylase by the substitution of one reagent. The new enzymatic method, compared to existing methods for the detection of phosphate on electrophoretic gels, is advantageous due to its sensitivity and generation of a nondiffusible formazan chromogenic product.",

keywords = "electrophoresis, isozymes, phosphate",

author = "Klebe, {Robert J.} and Sindi Schloss and Lisa Mock and Link, {Carol R.}",

year = "1981",

month = oct,

doi = "10.1007/BF00504257",

language = "English (US)",

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T1 - Visualization of isozymes which generate inorganic phosphate

AU - Klebe, Robert J.

AU - Schloss, Sindi

AU - Mock, Lisa

AU - Link, Carol R.

PY - 1981/10

Y1 - 1981/10

N2 - A sensitive, new enzymatic method for the detection of isozymes which liberate inorganic phosphate (or pyrophosphate) is described. The new method for the detection of phosphate differs from the established method for nucleoside phosphorylase by the substitution of one reagent. The new enzymatic method, compared to existing methods for the detection of phosphate on electrophoretic gels, is advantageous due to its sensitivity and generation of a nondiffusible formazan chromogenic product.

AB - A sensitive, new enzymatic method for the detection of isozymes which liberate inorganic phosphate (or pyrophosphate) is described. The new method for the detection of phosphate differs from the established method for nucleoside phosphorylase by the substitution of one reagent. The new enzymatic method, compared to existing methods for the detection of phosphate on electrophoretic gels, is advantageous due to its sensitivity and generation of a nondiffusible formazan chromogenic product.

KW - electrophoresis

KW - isozymes

KW - phosphate

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AN - SCOPUS:0019850575

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JO - Biochemical Genetics

JF - Biochemical Genetics

IS - 9-10

ER -

Visualization of isozymes which generate inorganic phosphate

Abstract

Keywords

ASJC Scopus subject areas

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