Visualization of isozymes which generate inorganic phosphate

Robert J. Klebe, Sindi Schloss, Lisa Mock, Carol R. Link

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


A sensitive, new enzymatic method for the detection of isozymes which liberate inorganic phosphate (or pyrophosphate) is described. The new method for the detection of phosphate differs from the established method for nucleoside phosphorylase by the substitution of one reagent. The new enzymatic method, compared to existing methods for the detection of phosphate on electrophoretic gels, is advantageous due to its sensitivity and generation of a nondiffusible formazan chromogenic product.

Original languageEnglish (US)
Pages (from-to)921-926
Number of pages6
JournalBiochemical Genetics
Issue number9-10
StatePublished - Oct 1981
Externally publishedYes


  • electrophoresis
  • isozymes
  • phosphate

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Biochemistry
  • Molecular Biology
  • Genetics


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