Abstract
The visualization and quantification of mitochondria-associated proteins with high power microscopy methods is of particular interest to investigate protein architecture in this organelle. We report the usage of a custom-made STimulated Emission Depletion (STED) fluorescence nanoscope with ~. 30. nm lateral resolution for protein mapping of Percoll-purified viable mitochondria from murine heart. Using this approach, we were able to quantify and resolve distinct protein clusters within mitochondria; specifically, cytochrome c oxidase subunit 2 is distributed in clusters of ~. 28. nm; whereas the voltage dependent anion channel 1 displays three size distributions of ~. 33, ~. 55 and ~. 83. nm.
Original language | English (US) |
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Pages (from-to) | 230-236 |
Number of pages | 7 |
Journal | Mitochondrion |
Volume | 12 |
Issue number | 2 |
DOIs | |
State | Published - Mar 2012 |
Externally published | Yes |
Keywords
- Cox2
- Imaging
- Mitochondria
- STED
- Subdiffraction-resolution
- VDAC1
ASJC Scopus subject areas
- Molecular Medicine
- Molecular Biology
- Cell Biology