Visualization and quantification of cardiac mitochondrial protein clusters with STED microscopy

Harpreet Singh, Rong Lu, Pedro Felipe Gardeazábal Rodríguez, Yong Wu, Jean C Bopassa, Enrico Stefani, Ligia Toro

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The visualization and quantification of mitochondria-associated proteins with high power microscopy methods is of particular interest to investigate protein architecture in this organelle. We report the usage of a custom-made STimulated Emission Depletion (STED) fluorescence nanoscope with ~. 30. nm lateral resolution for protein mapping of Percoll-purified viable mitochondria from murine heart. Using this approach, we were able to quantify and resolve distinct protein clusters within mitochondria; specifically, cytochrome c oxidase subunit 2 is distributed in clusters of ~. 28. nm; whereas the voltage dependent anion channel 1 displays three size distributions of ~. 33, ~. 55 and ~. 83. nm.

Original languageEnglish (US)
Pages (from-to)230-236
Number of pages7
JournalMitochondrion
Volume12
Issue number2
DOIs
StatePublished - Mar 1 2012
Externally publishedYes

Fingerprint

Mitochondrial Proteins
Microscopy
Voltage-Dependent Anion Channel 1
Mitochondria
Proteins
Heart Mitochondria
Electron Transport Complex IV
Organelles
Fluorescence

Keywords

  • Cox2
  • Imaging
  • Mitochondria
  • STED
  • Subdiffraction-resolution
  • VDAC1

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Cell Biology

Cite this

Visualization and quantification of cardiac mitochondrial protein clusters with STED microscopy. / Singh, Harpreet; Lu, Rong; Rodríguez, Pedro Felipe Gardeazábal; Wu, Yong; Bopassa, Jean C; Stefani, Enrico; Toro, Ligia.

In: Mitochondrion, Vol. 12, No. 2, 01.03.2012, p. 230-236.

Research output: Contribution to journalArticle

Singh, Harpreet ; Lu, Rong ; Rodríguez, Pedro Felipe Gardeazábal ; Wu, Yong ; Bopassa, Jean C ; Stefani, Enrico ; Toro, Ligia. / Visualization and quantification of cardiac mitochondrial protein clusters with STED microscopy. In: Mitochondrion. 2012 ; Vol. 12, No. 2. pp. 230-236.
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