Visualization and quantification of cardiac mitochondrial protein clusters with STED microscopy

Harpreet Singh, Rong Lu, Pedro Felipe Gardeazábal Rodríguez, Yong Wu, Jean Chrisostome Bopassa, Enrico Stefani, Ligia Toro

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

The visualization and quantification of mitochondria-associated proteins with high power microscopy methods is of particular interest to investigate protein architecture in this organelle. We report the usage of a custom-made STimulated Emission Depletion (STED) fluorescence nanoscope with ~. 30. nm lateral resolution for protein mapping of Percoll-purified viable mitochondria from murine heart. Using this approach, we were able to quantify and resolve distinct protein clusters within mitochondria; specifically, cytochrome c oxidase subunit 2 is distributed in clusters of ~. 28. nm; whereas the voltage dependent anion channel 1 displays three size distributions of ~. 33, ~. 55 and ~. 83. nm.

Original languageEnglish (US)
Pages (from-to)230-236
Number of pages7
JournalMitochondrion
Volume12
Issue number2
DOIs
StatePublished - Mar 2012
Externally publishedYes

Keywords

  • Cox2
  • Imaging
  • Mitochondria
  • STED
  • Subdiffraction-resolution
  • VDAC1

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Cell Biology

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